一种提取小型昆虫总RNA的有效方法
An effective method to extract total RNA from small insects
邓 顺;张友军;褚 栋
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作者单位：中国农业科学院蔬菜花卉研究所 北京 100081
中文关键词：小型昆虫，RT-PCR，RNA提取
英文关键词：Small insects; RT-PCR; RNA extraction
中文摘要：实验采用SDS，乙酸钾（KAc）等常规化学试剂从蚜虫等小型昆虫中获得完整且纯度较高的RNA。进一步的实验证明提取的RNA可用于RT-PCR以及cDNA文库的建立等。该方法简捷、相对安全和快速、提取效率较高，适合于中小型昆虫RNA的提取。
英文摘要：Integrated and purified RNA was extracted from aphids and other small insects, using ordinary chemicals, sodium dodecylsulfate (SDS), potassium acetate(KAc), etc. Experiments proved the extracted RNA had good quality that could be used to RT-PCR and const