30K蛋白对家蚕细胞及幼虫存活的作用
Influence of 30K protein in Bombyx mori on survival BmN cells and larvae
唐 平1，2**;李 兵3;沈卫德3; 陈劲频1;王文兵1**
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DOI：
作者单位：1.江苏大学生命科学研究院 镇江 212013； 2.江苏科技大学生物与环境工程学院 镇江 2120183苏州大学生命科学研究院 苏州 215123
中文关键词：家蚕；30K蛋白；杆状病毒；表达；生长
英文关键词：Bombyx mori；30K protein； baculovirus； expression； longevity
中文摘要：利用杆状病毒表达系统在家蚕Bombyx mori L.细胞BmN中表达家蚕30K蛋白，以亲本病毒BmPAK6为对照，将重组病毒Bm/r-30K分别感染BmN细胞及家蚕幼虫，观察其感染后不同时间的凋亡及存活率。与对照相比，重组病毒感染后的BmN细胞的存活率明显高于对照组，并且感染的家蚕幼虫存活时间也较长,表明体内过量表达家蚕30K蛋白有助于延长其细胞及幼虫的存活。
英文摘要：
The 30K protein was successfully expressed in the Bombyx mori L. (Bm) cells by baculovirus system. B. mori cells and larvae were infected with the recombinant baculovirus(Bm/r-30K), respectively. The viability of cells and larvae were assayed at various times post-infection and BmPAK6 was used as a control. The viability of BmN cells infected with baculovirus(Bm/r-30K) was higher than that of the cells infected with BmPAK6 virus, the viability and surviving time of B. mori larvae were increased as well. The results indicate that over-expression of the B. mori 30K gene contributes to increase the surviving time of BmN cells and larvae.