中国常见仓储书虱PCR-RFLP 快速识别
Rapid discrimination of four common species of store booklice, Liposcelis, from China using PCR-RFLP
秦 萌；李志红**；孙 晓；康芬芬；伍祎
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DOI：
作者单位：中国农业大学农学与生物技术学院 北京 100094
中文关键词：PCR-RFLP, 书虱, mtDNA, 快速识别
英文关键词：PCR-RFLP, booklice, mtDNA, rapid discrimination
中文摘要：应用PCR-RFLP 技术, 对我国4 种常见仓储书虱即嗜虫书虱Liposcelis entomop hila( Enderlein) 、嗜卷书虱L . bostry chophila( Badonnel) 、无色书虱L . decolor ( Pearman) 和小眼书虱L. paeta ( Pearman) 开展快速识别方法的研究。采用CTAB 法从上述4 种书虱体内提取基因组DNA, 选用1 对引物扩增16S rDNA 基因区域, 分别用5 种限制性内切酶对PCR 产物进行酶切。结果表明, PCR 扩增片段大小约为500 bp, 用限制性内切酶Dra I 酶切得到的片段可将供试书虱区分开。该方法不受4 种供试书虱地理种群、虫态( 成虫、若虫) 和性别的影响, 可用于4 种书虱的快速识别。
英文摘要：Rapid discrimination of four common species of store booklice, Liposcelis entomophila ( Enderlein) , L. bostrychop hila( Badonnel) , L. decolor ( Pearman) and L . p aeta Pearman was conducted by using PCR-RFLP. Genomic DNA was extracted from booklice with CTAB. Genes of 16S rRNA were amplified with specific primers and PCR products were digested with 5 restriction endonucleases respectively. The results showed that the PCR product was estimated to be about 500 bp in length, which could be digested by DraI . All the booklices examined could be discriminated according to the relevant banding patterns. The results were not affected by geographical populations, developmental stages and sexes of the four species. It is suggested that this method is reliable for rapid discrimination of the four booklice species.