溴氰菊酯对飞蝗羧酸酯酶基因表达的影响
Effect of deltamethrin on carboxylesterase gene expressionin Locusta migratoria
张建琴** 王 燕 李大琪 杨美玲 张建珍 马恩波***
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作者单位：山西大学应用生物学研究所 太原 030006
中文关键词：飞蝗，溴氰菊酯，羧酸酯酶，基因表达，Real-time PCR
英文关键词：Locusta migratoria, deltamethrin, carboxylesterase, gene expression, real-time PCR
中文摘要： 【目的】 研究溴氰菊酯作用下飞蝗羧酸酯酶基因的mRNA表达特性，为溴氰菊酯的代谢解毒及飞蝗Locusta migratoria防治中抗性风险的评估提供基础资料。【方法】 本文采用不同剂量溴氰菊酯处理3龄飞蝗，提取总RNA，体外反转录合成cDNA模板，采用Real-time PCR技术分析飞蝗羧酸酯酶基因在溴氰菊酯不同浓度和不同时间处理后的表达模式。【结果】 飞蝗经不同浓度溴氰菊酯处理12 h后，LmCesA3和LmCesE1表现为诱导效应；除LmCesA2外，其余羧酸酯酶基因经溴氰菊酯LD30剂量处理后分别在不同的时间点表现为诱导效应。【结论】 5个羧酸酯酶基因LmCesA1、LmCesA3、LmCesD1、LmCesE1和LmCesI1可以被溴氰菊酯诱导，表明其可能参与飞蝗对溴氰菊酯的代谢解毒及抗性产生。
英文摘要： [Objectives] To provide a theoretical basis for detecting deltamethrin detoxification and resistance risk assessment in Locusta migratoria by analyzing mRNA expression of carboxylesterase genes in response to deltamethrin exposure. [Methods] Locusts were exposed to different doses of deltamethrin and treated with deltamethrin for different periods of time in order to analyze the mRNA expression of carboxylesterase genes in response to deltamethrin. cDNAs were synthesized from the total RNA of different templates using reverse transcriptase. Real-time PCR was performed to analyze the mRNA expression of carboxylesterase genes. [Results] Exposureto different doses deltamethrin for 12 h induced the mRNA expression of LmCesA3 and LmCesE1. Except for LmCesA2, the transcription levels of the remaining carboxylesterase genes in third-instar nymphs of L. migratoria exposed to deltamethrin at LD30 were induced after different periods of deltamethrin exposure. [Conclusion] Deltamethrin induced mRNA expression in 5 carboxylesterase genes, including LmCesA1, LmCesA3, LmCesD1, LmCesE1 and LmCesI1. These results suggest that these genes play an important role in the detoxification of deltamethrin and the development of resistance to this pesticide in L. migratoria.