【目的】 筛选5龄飞蝗不同发育时间的最适内参基因，为相关研究提供基础数据。【方法】 本文选取β-肌动蛋白（β-actin）、延长因子（EF-1α）、3-磷酸甘油醛脱氢酶（GAPDH）、核糖体蛋白49（RP49）、α-微管蛋白（α-Tubulin）和18S核糖体RNA（18S rRNA）基因作为候选内参基因，运用实时定量PCR（qPCR）方法研究各基因在5龄飞蝗不同发育时间的相对表达量，用geNorm与Normfinder软件分析这6个基因表达稳定性。【结果】 geNorm分析结果显示6个内参基因表达稳定度M值顺序为：β-actin（0.3720）＞RP49（0.3750）＞α-Tubulin（0.4030）＞18S rRNA（0.4270）＞EF-1α（0.4970）＞GAPDH（0.6040）。M值越小表示基因表达稳定度越高，同时geNorm软件以标准化因子配对差异值（Pairwise variations）0.15默认为取舍值，由于V2/3=0.098＜0.15，所以最适内参基因数目为2个。运用NormFinder软件也得出相似的结果。【结论】 β-actin与RP49为5龄飞蝗不同发育时间的最适内参基因。

      [Objectives]  To identify the most appropriate reference genes on different days during the development of fifth-instar nymphs of Locusta migratoria to pave the way for relevant studies. [Methods]  Six genes were selected as candidate reference genes, including the beta actin gene (β-actin), the Elongation factor 1α gene (EF-1α), the Glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH), the Ribosomal protein 49 gene (RP49), the Alpha tubulin gene (α-Tubulin) and the 18S ribosome RNA gene (18S rRNA). The relative expression of these genes on different days during the development of L. migratoria was detected with quantitative real-time PCR (qPCR). The expression stability of these genes was then investigated using geNorm and Normfinder software. [Results]  Based on the results of geNorm analysis, the average expression stability (M) of the six genes was, in descending order; β-actin (0.3720)＞RP49 (0.3750)＞α-Tubulin (0.4030)＞18S rRNA (0.4270)＞EF-1α (0.4970)＞GAPDH (0.6040). The less the M value the more stable the gene expression. The software took 0.15 as a constant value for pairwise variations. The appropriate number of reference genes was 2 because V2/3=0.098＜0.15. Similar results were obtained using NormFinder software. [Conclusion]  β-actin and RP49 are the most suitable reference genes during the development of fifth-instar nymph of L. migratoria.