感染球孢白僵菌的烟粉虱若虫免疫应答转录组分析
Transcriptome analysis of Bemisia tabaci nymphs infected with Beauveria bassiana
王登杰** 吴圣勇 雷仲仁 王海鸿***
点击：2046次 下载：48次
DOI：10.7679/j.issn.2095-1353.2015.004
作者单位：中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室，北京 100193
中文关键词：白僵菌，侵染，烟粉虱，转录组
英文关键词： Beauveria bassiana, infect, Bemisia tabaci, transcriptome
中文摘要：
【目的】 筛选烟粉虱Bemisia
tabaci (Gennadius)若虫应对白僵菌Beauveria
bassiana侵染的应答基因，以进一步研究烟粉虱免疫反应的分子机制。【方法】 采用新一代高通量测序技术对感染和非感染白僵菌的烟粉虱4龄若虫进行了测序分析，并筛选了差异表达基因；利用生物信息学工具对转录组测序得到的基因进行了功能注释、分类以及参与的信号通路展示。【结果】 组装得到非冗余Unigene
232 554个，其N50和N90分别为1 153 bp和260 bp，平均长度为67 424
bp。对所有的基因进行差异表达分析发现：以P<0.05为标准筛选得到了1 166个差异表达基因，其中上调表达基因有474个，下调表达基因有692个， 其中，与免疫相关的基因有405个；GO富集分析发现：有416个GO
term有富集现象，包括156个生物学过程(66 402个Unigenes)，89 个细胞组分(27 645个 Unigenes) 和 154个分子功能 (73 417 个Unigenes)；KEGG代谢通路分析将烟粉虱转录组1 145个DEG匹配到309个通路上，其中76个通路得到了富集。【结论】 对405个可能参与到烟粉虱若虫对白僵菌侵染的免疫识别和防御的基因进行了测序。研究结果为从分子水平上开展应用虫生真菌防治烟粉虱的研究奠定信息学基础。
英文摘要：
[Objectives] To screen the whitefly Bemisia tabaci (Gennadius) genes that respond to infection by Beauveria bassiana in order to further elaborate molecular mechanisms underlying the immune response to fungal infection. [Methods] High throughput sequencing technology was used in the transcriptome analysis of Bemisia tabaci 4th instar nymphs that were either infected, or uninfected (control), with B. bassiana. Differentially expressed genes (DEGs) and their functions, classifications and signaling pathways were analyzed using bioinformatic tools. [Results] 232 554 non-redundant Unigenes were identified, among which N50 and N90 were 1 153 bp and 260 bp in length, respectively. The average length of all Unigenes were 674 24 bp. Among the 1 166 DEGs (P<0.05) identified, 474 were up-regulated and 692 were down-regulated, and 405 are known to be involved in insect immune response. The identified Unigenes were enriched in 156 biological processes (66 402 Unigenes), 89 cellular components (27 645 Unigenes) and 154 molecular functions (73 417 Unigenes) by GO analysis. KEGG pathway analysis indicated that 1 145 DEGs were mapped to 309 distinctive pathways, and were enriched in 76 pathways. [Conclusion] Four hundred and five genes that are possibly involved in the immune recognition and defense of whitefly nymphs against B. bassiana infection were sequenced. The results provide a bioinformatic data base for using entomopathogenic fungi as a biological control for whitefly.