烟粉虱内共生菌Rickettsia在植物体内的分布及转移效率初探
Preliminary research on the distribution and transmission efficiency of Rickettsia, an endosymbiont of whitefly Bemisia tabaci
安 璇** 李翌菡 李绍建 郭长飞 任顺祥 邱宝利***
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DOI：10.7679/j.issn.2095-1353.2015.014
作者单位：华南农业大学昆虫学系，生物防治教育部工程研究中心，广州 510640
中文关键词：烟粉虱，内共生菌，Rickettsia，水平传播，寄主植物
英文关键词： Bemisia tabaci, endosymbiont, Rickettsia, horizontal transmission, host plant
中文摘要：
【目的】 检测Q型烟粉虱Bemisia tabaci （Gennadius）体内Rickettsia的感染情况，研究分析Rickettsia共生菌经烟粉虱传入豇豆植物后的分布、转移效率等。【方法】 以Q型烟粉虱为实验材料，利用常规PCR及荧光原位杂交技术(FISH)，检测了烟粉虱体内Rickettsia的感染率，以及Rickettsia传入豇豆植物体内后的存留情况。【结果】 Q型烟粉虱可以通过取食将Rickettsia传至豇豆植株内；接虫数量与Rickettsia传入效率及其在取食部位相邻的下部叶片中检测到的起始时间呈负相关；Rickettsia经烟粉虱取食传入豇豆叶片后，集中分布在叶片的韧皮部筛管中；基于16S rRNA的系统发育分析结果表明，Q型烟粉虱体内的Rickettsia与经取食传入豇豆叶片的Rickettsia高度同源。【结论】 Rickettsia可以通过烟粉虱的取食传入植物体内，并且可以在相邻叶片之间转移传播，Rickettsia在由寄主昆虫向植株传播过程中高度保守。
英文摘要：
[Objectives] To detect the infection of Bemisia tabaci by Rickettsia, and the distribution and dissemination of Rickettsia after its transmission to cowpea leaves through whitefly feeding. [Methods] Infection of the B. tabaci Q biotype by Rickettsia, and its persistence in cowpea plants, were detected by PCR and fluoresent in situ hybridization (FISH). [Results] The distribution of Rickettsia was limited to the phloem vessels of cowpea leaves, Rickettsia could move along the vessels between different leaves, and its efficiency of dissemination was highly related to the initial population size of Rickettsia-positive B. tabaci individuals that fed on the cowpea leaves. Phylogenetic analysis based on 16S rRNA variation showed that Rickettsia in cowpea plants and whiteflies was 100% identical. [Conclusion] Rickettsia can be horizontally transmitted to cowpea plants through the feeding of the B. tabaci Q biotype and little genetic variation appears to be associated with horizontal transmission from insect to host plants.