转Bt基因水稻“赣绿1号”对非靶标害虫褐飞虱取食、产卵行为及生长发育的影响
Effect of the transgenic Bt rice variety “Ganlv 1” on the feeding, oviposition behavior and development of Nilaparvata lugens (Stal)
徐雪亮1** 肖叶青2 季香云3 刘子荣1 陈连生4 蒋杰贤3陈大洲2 胡兰香2 姚英
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DOI：10.7679/j.issn.2095-1353.2015.117
作者单位：1. 江西省农业科学院农业应用微生物研究所，南昌 330200；2. 江西省农业科学院水稻研究所，南昌 330200；3. 上海市农业科学院生态环境保护研究所，上海 201403；4. 江西东乡县农业技术推广中心，抚州 331800
中文关键词：转Bt基因水稻，褐飞虱，取食行为，产卵行为，生长发育
英文关键词：transgenic Bt rice, Nilaparvata lugens, feeding behavior, oviposition behavior, development
中文摘要：【目的】 研究转cry1Ab/Ac基因水稻“赣绿1号”对非靶标害虫褐飞虱Nilaparvata lugens的潜在影响。【方法】 采用标准苗期集团筛选法研究“赣绿1号”对褐飞虱的抗性；利用取食和产卵选择性研究“赣绿1号”对褐飞虱取食、产卵行为的影响；用“赣绿1号”连续饲养褐飞虱5代，研究对褐飞虱生长发育（若虫发育历期、成虫体重）和繁殖（孵化子代若虫数量）的影响。【结果】 “赣绿1号”与非转基因亲本对褐飞虱均表现为中抗；褐飞虱于“赣绿1号”上取食、产卵选择与非转基因亲本均无显著差异；除取食“赣绿1号”第2代褐飞虱第3龄龄期显著短于取食非转基因亲本水稻的褐飞虱外，第2代和第5代若虫各龄龄期均无显著差异，褐飞虱雌雄成虫体重和孵化若虫数均无显著差异。【结论】 与对照水稻相比，转Bt基因水稻“赣绿1号”对非靶标害虫褐飞虱的苗期抗性、取食、产卵行为及生长发育等无明显的不利影响。
英文摘要： [Objectives] To assess the potential impact of the transgenic cry1Ab/Ac rice variety “Ganlv 1” on the brown planthopper (BPH). [Methods] The resistance of “Ganlv 1” and non-transgenic control rice to BPH was compared using the Standard Seedbox Screening Technique (SST). The feeding and oviposition behavior of BPH on “Ganlv 1” and control rice were measured by conducting a feeding and oviposition selectivity experiment. The effects of “Ganlv 1” on development (nymph duration and adult weight) and reproduction (number of offspring) of BPH were evaluated by feeding five successive BPH generations on this variety. [Results] Both “Ganlv 1” and non-transgenic rice showed moderate resistance to BPH at the seedling stage. There were no significant differences in the feeding or oviposition preferences of BPH for “Ganlv 1” vs the non-transgenic strain. No significant differences in nymph duration, adult weight and number of 2nd and 5th generation offspring were found between BPH fed on “Ganlv 1” and the non-transgenic strain, except that the duration of the 3rd instar of the 2nd generation fed on “Ganlv 1” was shorter than that fed on non-transgenic rice. [Conclusion] There was no obvious adverse effect on the resistance of seedlings to BPH between the transgenic Bt rice “Ganlv 1” and a non-transgenic strain, nor were there significant differences in the feeding and oviposition preferences, or development, of BPH fed on “Ganlv 1” compared to those fed on the non-transgenic strain.