韭菜迟眼蕈蚊体内及韭菜根际微生物的多样性分析
Microbial diversity of Bradysia odoriphaga and the rhizosphere microbes of chives
高欢欢1** 翟一凡1 王海艳1, 2 云 雷1 覃冬云1 于 毅1***
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DOI：10.7679/j.issn.2095-1353.2015.121
作者单位：1．山东农业科学院植物保护研究所，济南 250100；2．中国农业大学农学与生物技术学院，北京 100193
中文关键词：韭蛆，韭菜，微生物，多样性
英文关键词：Bradysia odoriphaga, chives, microorganism, diversity
中文摘要：【目的】 为了了解韭菜迟眼蕈蚊Bradysia odoriphaga生存环境中微生物的多样性。【方法】 本研究对韭蛆、韭菜鳞茎、根部以及土壤中的微生物进行分离纯化，对部分代表性细菌进行了生理生化特征的分析，并对分离得到的真菌及细菌分别采用ITS和16S rDNA基因分析的方法进行鉴定。【结果】 总共分离鉴定出25种微生物，在韭菜鳞茎和土壤中分别分离出半知菌亚门（Deuteromycotina）的球孢白僵菌Beauveria bassiana和球孢虫草Cordyceps bassiana两种真菌，在韭蛆体内、韭菜鳞茎、韭菜根部以及土壤中分别分离出10、15、9、6种细菌，以变形菌门（Proteobacteria）和厚壁菌门（Firmicutes）细菌为主，其余的细菌分别属于放线菌门（Actinobacteria）和拟杆菌门（Bacteroidetes）。【结论】 本研究可以为寻找韭菜、韭蛆和微生物三者之间的协同进化关系提供理论基础，并期望为韭蛆的共生防治提供指导。
英文摘要： [Objectives] To understand microbial diversity in Bradysia odoriphaga and its environment. [Methods] Microorganisms in B. odoriphaga, and in the bulbs and roots of chives, and soil were separated, purified, and identified through ITS and 16S rDNA genetic analysis. Physiological and biochemical analyses was then carried out for some representative bacteria. [Results] 25 microbial species were identified from the environment of B. odoriphaga. Beauveria bassiana and Cordyceps bassiana (Deuteromycotina), were found in both chive bulbs and soil. Most bacterial species belonged to the Proteobacteria and Firmicutes，the rest belonged to Actinobacteria and Bacteroidetes. 10, 15, 9, 6 bacterial species were identified in B. odoriphaga, and in the chive bulbs, roots and soil, respectively. [Conclusion] This study provides a theoretical foundation for investigating coevolution among chives, B. odoriphaga and microorganisms. We hope that it will also contribute to the symbiotic control of B. odoriphaga.