【目的】 韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang是为害韭菜等蔬菜的重要害虫，规模化饲养提供大量虫源是其他研究工作得以开展的基础。本研究旨在解决目前韭菜迟眼蕈蚊饲养规模小、材料和劳动力成本高等问题。【方法】 本实验室利用土豆、花生、大豆为食物进行了规模化饲养韭菜迟眼蕈蚊的研究，测定了取食上述饲料的韭菜迟眼蕈蚊的成活率、繁殖力、羽化节律和性比。【结果】 取食土豆、花生、大豆等食物的韭菜迟眼蕈蚊的成活率和繁殖力与取食人工饲料和韭菜的无显著差异，卵到成虫的发育历期为20~23 d，雌雄性比为0.8︰1~1.2︰1。在规模化饲养过程中，土豆、花生、大豆等饲料有发霉长菌现象，经分子鉴定，主要种类为雅致放射毛霉、巴克斯毛霉、黄曲霉和赭曲霉，均为腐生菌，不影响韭菜迟眼蕈蚊的存活和繁殖。【结论】 该技术省工省时，成本低，特别适合实验室种群的维持和大量试虫的饲养。

    [Objectives]  Bradysia odoriphaga Yang et Zhang is an important pest of Chinese chives. Large scale rearing of this pest is useful for developing methods to control it. Our study addressed the high material and labor costs, and relatively small number of animals produced, by the present rearing technique. [Methods]  We experimentally reared B. odoriphaga larvae on potatoes, peanuts and beans and compared the survival rate, fecundity, emergence rhythm and sex ratio of B. odoriphaga reared on this diet to those reared on Chinese chives and artificial diets. [Results]  There was no significant difference between the survival rate and fecundity of B. odoriphaga larvae reared on the new diet and those reared on Chinese chives and artificial diets. The developmental period from egg to adult of larvae reared on the new diet was 20-23 days and the sex ratio was 0.8︰1-1.2︰1. Although the new diet became easily infected with fungi, molecular identification revealed that the main fungal species were Actinomucor elegans, Backusella circina, Aspergillus flavus and Aspergillus ochraceus, all of which are saprophytic and did not affect the survival and breeding of B. odoriphaga. [Conclusion]  Our technique saved time, labor and materials, and is especially suitable for the maintenance of laboratory population and mass rearing.