韭菜迟眼蕈蚊Bradysia odoriphaga 嗅觉行为反应
Olfactory behavioral responses of Bradysia odoriphaga
徐 蕾** 赵彤华 刘培斌 钟 涛 王 哲 许国庆***
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DOI：10.7679/j.issn.2095-1353.2016.147
作者单位：辽宁省农业科学院植物保护研究所，沈阳 110161
中文关键词：韭菜迟眼蕈蚊，引诱率，“Y”型嗅觉仪，四臂嗅觉仪，行为反应
英文关键词： Bradysia odoriphaga, attraction rate, “Y” tube olfactory, four-arm olfactometer, behavioural responses
中文摘要：
【目的】 韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang是韭菜生产中最主要的害虫。通过嗅觉趋性试验测定了3龄幼虫和雌虫对多种味源植物的寄主选择性及行为反应，为进一步研发绿色高效的生态防控技术提供理论依据。【方法】 利用“Y”型嗅觉仪测定幼虫对健康韭菜、灰霉菌侵染韭菜、平菇、大葱和小白菜等几种寄主植物的嗅觉趋性反应，利用四臂嗅觉仪测定雌虫对健康韭菜、机械损伤韭菜、腐殖质和浸泡大豆4种味源材料的行为反应。【结果】 “Y”型嗅觉仪测定结果表明健康韭菜、平菇和大葱对3龄幼虫的引诱作用较强；四臂嗅觉仪测定结果表明韭菜迟眼蕈蚊雌虫对机械损伤韭菜和腐殖质表现出较强趋性。【结论】 应用嗅觉仪观测了韭菜迟眼蕈蚊3龄幼虫、雌虫对不同味源材料在不同条件下的定向选择行为，此项工作可为韭菜迟眼蕈蚊植物源引诱剂或驱避剂的进一步研发奠定基础。
英文摘要：
[Objectives] To provide a theoretical and practical basis for developing effective ecological control methods for Bradysia odoriphaga, the main insect pest of Chinese chives. [Methods] The preferences of 3rd instar larvae for several host plant species were tested in a “Y” tube olfactometer, and the behavioral responses of females to different host plant volatiles were tested in a four-arm olfactometer. [Results] The results of the “Y” tube olfactometer test showed that larvae were significantly attracted by intact Chinese chives, oyster mushrooms and spring onions. Females were most attracted to mechanically damaged Chinese chives and humus. [Conclusion] The results of this study provide some valuable insights for the development of botanical pheromones for the control of Bradysia odoriphaga.