【目的】 本研究主要目的是通过染色饲料饲喂获得标记的二化螟Chilo suppressalis（Walker）成虫，且不影响其生理和行为，为二化螟成虫的扩散和运动行为提供一种标记方法。【方法】 笔者所在实验室前期筛选出一个适合二化螟染料及浓度，通过连续3代饲养观察染色剂对二化螟标记效果及生长发育的影响。【结果】 连续3代的幼虫期饲喂含500 mg·kg^-1浓度的苏丹红7B人工饲料后，幼虫、成虫及卵均可被染色，饲喂染色饲料后，其化蛹率、羽化率、成虫产卵量、寿命和卵孵化率与对照均没有显著差异。取食染色饲料的二化螟雌、雄平均蛹重比对照分别高5.3 mg和4.2 mg，雌、雄平均幼虫+蛹历期比对照延长2.8 d和2.3 d。【结论】 该标记技术对二化螟没有明显的生物学影响，操作简便且经济可行，是一种较好的大规模标记二化螟的方法。

[Objectives]  To develop a diet that can mark Chilo suppressalis without significant adverse effects. The ability to mark individual insects would be useful for studying behaviors such as adult dispersal, mate choice and food preferences. [Methods]  500 mg·kg^-1 Sudan Red 7B was incorporated into an artificial diet fed to C. suppressalis larvae and biological parameters of marked individuals were recorded and compared to those of a control group fed the same artificial diet without Sudan Red 7B. [Results]  The diet containing Sudan Red 7B effectively marked eggs, larvae, and adults, and the pupation rate, emergence rate, fecundity, egg hatching rate and adult longevity of the Sudan Red (SR) treatment group were similar to those of the control. Interestingly, the pupal weights of SR females and males were respectively 5.3 mg and 4.2 mg higher than those of their control group counterparts. In addition, the combined larval and pupal periods of SR females and males exceeded those of the controls by 2.8 d and 2.3 d, respectively. [Conclusion]  Our results indicate that incorporating 500 mg·kg^-1 Sudan Red 7B into an artificial diet can effectively mark C. suppressalis eggs, larvae, and adults without significant adverse effects. In addition, this method requires relatively less time and labor than other methods.