二化螟谷胱甘肽-S-转移酶基因的鉴定与表达模式分析
Identifying, and measuring the expression profiles, of Chilo suppressalis glutathione-S-transferase genes
金燕璐；张邦贤；林华峰
点击：685次 下载：7次
DOI：10.7679/j.issn.2095-1353.2018.046
作者单位：安徽农业大学植物保护学院；安徽农业大学植物保护学院；安徽农业大学植物保护学院
中文关键词：二化螟，谷胱甘肽-S-转移酶，序列分析，表达模式
英文关键词：Chilo suppressalis, glutathione-S-transferase genes, sequence analysis, transcriptional profiles
中文摘要：
摘要 【目的】 鉴定二化螟Chilo suppressalis 谷胱甘肽-S-转移酶（Glutathione-S-transferase，GST）基因，明确GST 基因在幼虫各组织中的表达谱，并分析GST 应对氯虫苯甲酰胺胁迫的表达模式。【方法】从二化螟转录组数据库中鉴定GST 的cDNA 序列，使用实时荧光定量PCR 技术分析GST 在幼虫不同组织内的相对表达水平，并考察亚致死剂量氯虫苯甲酰胺处理幼虫之后GST 表达水平的变化。【结果】 从二化螟转录组中检索得到16 个GST 基因，分别被命名为CsGSTd1-CsGSTu1。这16 个GST 被划分为6 个家族（Delta、Epsilon、Omega、Sigma、Zeta 和Theta）和一个“未分类”亚组（Unclassified subgroup）。CsGSTd2、CsGSTd3、CsGSTe1、CsGSTo3、CsGSTt1 主要表达于脂肪体，CsGSTe3 主要表达于马氏管。未检测到特异表达于中肠的GST 基因。氯虫苯甲酰胺处理6 h 后，CsGSTo2 显著上调，但CsGSTd2、CsGSTd3、CsGSTe3、CsGSTo1 和CsGSTt1 显著下调。药剂处理12 h 后，CsGSTd1、CsGSTd2、CsGSTd3、CsGSTe1、CsGSTe2、CsGSTe3、CsGSTo2、CsGSTo3、CsGSTo4 和CsGSTt1 显著上调，而CsGSTu1 显著下调。【结论】CsGSTd1、CsGSTd2、CsGSTd3、CsGSTe1、CsGSTe2、CsGSTe3、CsGSTo2、CsGSTo3、CsGSTo4 和CsGSTt1 可能参与了对氯虫苯甲酰胺的解毒代谢。
英文摘要：
Abstract [Objectives] To identify glutathione-S-transferase (GST) genes in Chilo suppressalis, determine their
expression profiles in various larval tissues, and analyze their
transcriptional profiles in response to chlorantraniliprole. [Methods] The cDNA sequences of GST genes were
identified from the C. suppressalis transcriptome database and their expression profiles in different larval
tissues investigated with real-time quantitative PCR. Variation in GST expression levels after exposure to
a sublethal dose of chlorantraniliprole was measured. [Results] A total of 16 GSTs were
identified and named CsGSTd1- CsGSTu1. These genes were classified into six families (Delta, Epsilon, Omega,
Sigama, Zeta, and Theta) and an “unclassified” subgroup. CsGSTd2、CsGSTd3、CsGSTe1、CsGSTo3、CsGSTt1 were mainly expressed in the fat-body, while CsGSTe3 was enriched in the malpighian tubes. No midgut-specific
genes were detected. CsGSTo2 was
significantly upregulated 6 h after exposure to chlorantraniliprole whereas CsGSTd2, CsGSTd3, CsGSTe3, CsGSTo1 and CsGSTt1 were significantly downregulated. CsGSTd1, CsGSTd2, CsGSTd3, CsGSTe1, CsGSTe2, CsGSTe3, CsGSTo2, CsGSTo3, CsGSTo4 and CsGSTt1 were significantly upregulated 12 h after
chlorantraniliprole exposure, but CsGSTu1 was significantly downregulated. [Conclusion] CsGSTd1, CsGSTd2, CsGSTd3, CsGSTe1, CsGSTe2, CsGSTe3, CsGSTo2, CsGSTo3, CsGSTo4 and CsGSTt1 may
play an important role in the detoxification of chlorantraniliprole.