【目的】 柑橘木虱Diaphorina citri Kuwayama是传播黄龙病病菌唯一的自然媒介昆虫，本文研究了黄龙病病菌在柑橘木虱各个发育阶段体内的分布以及感染动态，为有效防控柑橘木虱提供科学数据。【方法】 利用常规PCR检测柑橘木虱若虫体内的黄龙病病菌，利用qRT-PCR和荧光原位杂交技术分别检测黄龙病病菌在柑橘木虱不同发育虫态体内的含量与分布形式。【结果】 常规PCR可以在3-5龄若虫以及成虫体内检测到黄龙病病菌，而qRT-PCR除了3-5龄若虫外，还可以在2龄若虫体内检测到黄龙病病菌。卵和各龄期若虫中黄龙病病菌的含量是随着龄期的变大而不断增多。产卵盛期的成虫木虱体内黄龙病病菌的含量最高，显著高于产卵前期以及产卵后期。荧光原位杂交技术可以检测到4龄、5龄若虫及成虫体内的黄龙病病菌分布形态，病菌在若虫主要分布在U型含菌体内，而在雌雄成虫体内均是散布型分布。【结论】 柑橘黄龙病在柑橘木虱的2-5龄若虫及成虫中都有感染，但其含量与分布形式因发育阶段不同而有显著差异。

 [Objectives]  To provide additional scientific information for the management of Asian citrus psyllid (ACP) Diaphorina citri Kuwayama, the most important vector of the Huanglongbing (HLB) bacteria. [Methods]  The infection dynamics and distribution of HLB bacteria in different developmental stages of ACP were investigated using PCR, qRT-PCR and Fluorescence in situ hybridization (FISH). [Results]  HLB bacteria can be detected by normal PCR in 3^rd to 5^th instar nymphs and adults but can only be detected in 2^nd instar nymphs with qRT-PCR. The titer of HLB bacteria increased with nymph instar, and the titer of HLB bacteria in female adults in peak reproductive condition was significantly higher than that in pre-oviposition and post-oviposition females. In 4^th to 5^th instar nymphs, the distribution of HLB bacteria was scattered but mostly in the U-shaped bacteriome in the nymphs’ abdomen. The distribution of HLB bacteria in male and female adults was similar to that in nymphs, scattered rather than concentrated in the reproductive system. [Conclusion]  HLB bacteria infected 2-5 instar ACP nymphs and adults but its titer and distribution patterns varied among these different development stages.