宛氏拟青霉与球孢白僵菌对柑橘 木虱的致病力分析
Pathogenicity of Paecilomyces variotii and Beauveria bassiana to Diaphorina citri
宋晓兵；彭埃天；凌金锋；崔一平；程保平；张炼辉
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DOI：10.7679/j.issn.2095-1353.2018.079
作者单位：广东省农业科学院植物保护研究所，广东省植物保护新技术重点实验室，广州 510640； 华南农业大学农学院，广东省微生物信号与作物病害防控重点实验室，广州 510640
中文关键词： 宛氏拟青霉，球孢白僵菌，柑橘木虱，致病力，生物防治
英文关键词：Paecilomyces variotii, Beauveria bassiana, Diaphorina citri, pathogenicity, biocontrol
中文摘要： 【目的】 比较宛氏拟青霉WS-11和球孢白僵菌QB-28对柑橘木虱成虫的致病力，为生防制剂的田间应用提供理论支持。【方法】 采用孢子悬浮液浸没法比较研究了2种真菌对柑橘木虱的致病力，并利用时间-剂量-死亡率模型估计了2种真菌对柑橘木虱的致死剂量与致死时间。【结果】 宛氏拟青霉WS-11在孢子悬浮液浓度为1×108 spores/mL时，累计死亡率达到90.67%，球孢白僵菌QB-28则达到97.33%。时间-剂量-死亡率模型中Hosmer-Lemeshow方法拟合异质性检验表明模型拟合良好，在接种后9 d，宛氏拟青霉WS-11和球孢白僵菌QB-28对柑橘木虱的LC50分别为7.57×106 spores/mL和8.39×105 spores/mL；当孢子悬浮液浓度为1×108 spores/mL时，2种真菌对柑橘木虱的LT50分别为2.50 d和1.93 d。【结论】 宛氏拟青霉WS-11和球孢白僵菌QB-28对柑橘木虱均有较好的致病力，具有较好的生防潜质，其中球孢白僵菌对柑橘木虱的致病力高于宛氏拟青霉，致死效率更高。
英文摘要：
[Objectives] To compare the pathogenicity
of Paecilomyces variotii and Beauveria bassiana WS-11 and QB-28 strains to Diaphorina citri adults, and provide
theoretical support for the use of these biocontrol agents in the field. [Methods] The spore fluid immersion
method was used to compare the virulence of both kinds of fungi to D. citri adults. A time-dose- mortality
model was used to estimate lethal doses and the lethal period of exposure to each
fungus. [Results] The highest
concentration of P. variotii caused
90.67% mortality where as B. bassiana caused 97.33% mortality rate on centration of 1×108 spores/mL. The time-dose-mortality
model was a good fit to the data and could be used to estimate exposure time
and dose effects. The estimated LC50 values after 9 days inoculation
with P. variotii or B. bassiana were 7.57×106 spores/mL and 8.39×105 spores/mL,
respectively, whereas at a concentration of 1×108 spores/mL LT50 values were 2.50 d and 1.93 d, respectively. [Conclusion] Both
fungi were highly pathogenic to Diaphorina citri adults
and consequently should both be considered as potential biological control
agents for controlling this pest. The virulence of B. bassiana was higher than that of P. variotii, and it
consequently has greater
potential as a biological control than P. variotii.