【目的】 筛选柑橘木虱Diaphorina citri Kuwayama应对球孢白僵菌侵染的免疫应答及其网络调控基因，以进一步探讨柑橘木虱对球孢白僵菌的免疫防御机制。【方法】 采用Illumina高通量测序平台对感染球孢白僵菌24、48、72 h与健康的柑橘木虱转录组进行了测序，利用生物信息学软件对筛选到的差异表达基因进行了功能注释、分类以及参与的信号通路分析。【结果】 组装得到138 313条不可延长的非冗余Unigene，其N50和N90分别为2 532 bp和413 bp，平均长度为1 191.26 bp。在CK vs. S24h、CK vs. S48h和CK vs. S72h 3个转录组里分别获得了971、1 671和752个显著差异表达基因（DEGs），GO富集分析表明分别有405、614和542个DEGs显著富集到56、83和60个GO terms中，KEGG pathway分析结果显示分别有98、333和247个DEGs显著富集到10、27和25条代谢通路里。【结论】 差异表达基因主要富集在能量代谢、离子运输、转录和翻译调控、生殖和发育调控以及免疫防御反应等相关通路，大部分编码潜在的与免疫识别及调控相关的基因，并从中筛选到5个显著上调表达的免疫相关基因，为开展柑橘木虱免疫应答虫生真菌的研究奠定理论基础。下一步将进行免疫相关基因的q-PCR验证及表达量分析，研究其在柑橘木虱应对球孢白僵菌侵染过程中的作用。

 [Objectives]  To identify the network of regulatory genes involved in the immune response of Diaphorina citri to infection with Beauveria bassiana, and to further investigate the immune defense mechanism of D. citri to B. bassiana. [Methods]  An Illumina high-throughput sequencing platform was used to analyze the transcriptomes of infected and uninfected D. citri 24, 48 and 72 h after treatment. Differentially expressed genes and their functions, classifications and signaling pathways were analyzed using bioinformatic tools. [Results]  138 313 non-redundant Unigenes were identified, among which N50 and N90 were 2 532 bp and 413 bp in length, respectively. The average length of all Unigenes was 1 191.26 bp. 971, 1 671, 752 differentially expressed genes (DEGs) were obtained from the transcriptome data of CK vs. S24h, CK vs. S48h, and CK vs. S72h. 405, 614 and 542 DEGs were significantly differentially enriched in 56, 83 and 60 GO terms, respectively. KEGG pathway analysis indicated that 98, 333, and 247 expressed genes were significant differentially enriched in 10, 27, and 25 metabolic pathways, respectively. [Conclusion]  Differentially expressed genes are mainly enriched in related pathways, such as energy metabolism, ion transport, transcription and translation regulation, reproduction and development regulation, and immune defence response, and most encode potential genes related to immune recognition and regulation. We identified 5 significant up-regulations of these immune-related genes. These findings provide a theoretical foundation for the study of the immune response of D. citri to entomogenous fungi. We plan to next use q-PCR to verify the expression analysis of immune-related genes to further investigate their role inthe immune response of D. citri to Beauveria bassiana.