蜜蜂肠道菌群的培养方法及特性研究进展
Advances in research on honey bee gut microbiota, including anaerobic culturing methods
董志祥1** 李还原1 陈奕霏1 张棋麟1 李继莲2*** 郭 军1***
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DOI：10.7679/j.issn.2095-1353.2018.117
作者单位：1. 昆明理工大学生命科学与技术学院，昆明 650500； 2. 中国农业科学院蜜蜂研究所，农业部授粉昆虫生物学重点实验室，北京100093
中文关键词： 蜜蜂，肠道菌群，厌氧培养
英文关键词：honeybee, gut microbiota, anaerobic cultivation
中文摘要：
肠道菌群在蜜蜂的消化、营养和抗病性等方面发挥了很多潜在的益生作用。为了加深对以蜜蜂为主的传粉昆虫肠道菌群的了解，本文综述了蜜蜂肠道菌群的人工厌氧培养方法及特性，重点综述了 Snodgrassella属、Gilliamella属、Frischella属、Lactobacillus属、Bifidobacterium属和Alpha-1，Alpha-2厌氧细菌类群。旨在通过特定的培养方法获取纯培养的细菌，以供进一步研究特定肠道菌群与特定功能的直接联系。希望这些培养技术能帮助大家提升对蜜蜂肠道共生菌在蜜蜂营养和健康方面所起的作用有所了解。
英文摘要：
Gut microbiota have many potential beneficial effects on the digestion,
nutrition, resistance and health, of bees. To better understand the gut
microbiota of pollinators, this paper summarizes artificial cultivation methods
for, and biological characteristics of, honey bee gut microbiota, including the
bacterial genera Snodgrassella, Gilliamella, Frischella, Lactobacillus,
and Bifidobacterium, as well as two
anaerobic bacterial compositions, alpha-1 and alpha-2. This knowledge will help
isolate bacterial species for further research on their specific function in
the honey bee, an approach that will facilitate better understanding of the
role played by gut microbiota in bee nutrition and health.