中哈边境区域黑腿星翅蝗遗传多样性与遗传分化研究
Genetic diversity and genetic differentiation of Calliptamus barbarus in Sino-Kazakh border areas
麦季玮1** 徐 叶2 施 燚1 王添歆1ROMAN Jashenko3 赵 伟1
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DOI:10.7679/j.issn.2095-1353.2019.033
作者单位:1. 兰州大学生命科学学院,兰州 730000;2. 中亚区域跨境有害生物联合控制国际研究中心,新疆师范大学生命科学学院,乌鲁木齐 830054;3. 哈萨克斯坦阿勒法拉比大学,阿拉木图 050038
中文关键词:黑腿星翅蝗;线粒体DNA;细胞色素氧化酶亚基Ⅰ(COⅠ);地理种群,基因流
英文关键词:Calliptamus barbarus; mitochondrial DNA; COⅠ gene; geographic population; gene flow
中文摘要:【目的】 为了明确中哈边境区域黑腿星翅蝗Calliptamus barbarus Costa的遗传多样性,阐明其区域性发生的遗传机制。【方法】 测定中哈边境新疆边境区域阿勒泰、塔城、博乐、伊犁等和哈萨克斯坦境内黑腿星翅蝗5个种群100个体的COⅠ基因全长序列(1 540 bp),利用DnaSP 5.0,MEGA 6.0和Arlequin 3.5等软件分析种群遗传多样性与分化情况。【结果】 共获得100条COⅠ基因序列,在5个地理种群中发现122个变异位点,包含69个单倍型,其中1个单倍型为4个种群所共享。种群间的遗传距离范围为0.001-0.086。总群体单倍型多样性指数Hd= 0.987,各地理种群单倍型多样性介于0.973-0.995之间,总群体核苷酸多样性Pi=0.008 4,总群体的遗传分化系数Gst=0.004 5,总群体固定系数Fst=0.014 2,总基因流Nm=4.61。中性检验(Tajima¢s D=-1.553 3,P >0.05;Fu’s Fs=-3.732 4,P >0.05)结果表明中哈边境黑腿星翅蝗种群在较近的历史上没有出现群体扩张。单倍型网络图和NJ单倍型系统树结果一致,博乐种群与其他种群分化较明显,AMOVA分子变异分析结果表明中哈边境不同地理种群黑腿星翅蝗的遗传分化主要来自种群内部(98.56%),种群间变异水平很低(1.44%)。不同地理种群的遗传距离与地理距离间的相关性不显著。各单倍型散布在不同地理种群中,未形成明显的系统地理结构。【结论】 中哈边境区域不同地理种群黑腿星翅蝗基因交流频繁,遗传分化程度低。
英文摘要:
Abstract [Objectives] To investigate the genetic diversity of Calliptamus barbarus in the border area between China and Kazakhstan, and reveal the molecular mechanism underlying serious regional outbreaks of this pest. [Methods] Specimens of C. barbarus were collected from 5 different geographic populations (ALT, TC, BL, YL and Kazakhstan) on the border between China and Kazakhstan. A total of 100 full-length sequences of the mitochondrial cytochrome oxidase subunit I (COⅠ) gene (1 540 bp) were obtained, sequenced, and compared, using DnaSP 5.0 and Arlequin 3.5 software. [Results] About 122 mutations were found, including 69 haplotypes, one of which was shared by all 4 populations. The genetic distance between populations ranged from 0.001 to 0.086 and the total haplotype diversity index (Hd) was 0.987. The diversity of haplotypes among different geographic populations ranged from 0.973 to 0.995. The total nucleotide diversity (Pi), total genetic differentiation coefficient (Gst), total fixed coefficient (Fst), and total gene flow (Nm) were 0.008 4, 0.004 5, 0.014 2 and 4.61, respectively. Neutrality test results(Tajima′s D=-1.553 3,P >0.05;Fu’s Fs=-3.732 4,P >0.05) indicated that C. barbarus populations on the border between China and Kazakhstan have not recently expanded. The information obtained from the haplotype network and a neighbour-joining phylogenetic tree were consistent, and the differentiation of the BL population from other populations was obvious. The genetic difference between populations (1.44%) is much less than the genetic variation within populations, indicating that geographic distance has no obvious effect on the genetic distance between different populations. No obvious geographic structure is apparent in a phylogenetic tree and a haplotype network based on haplotypes of the different geographic populations. [Conclusion] There is frequent gene exchange between different geographic populations of C. barbarus in the border area between China and Kazakhstan and genetic differentiation of this species in this region is consequently low.