乙基多杀菌素和乙虫腈对西花蓟马解毒酶和乙酰胆碱酯酶活性的影响
Effects of spinetoram and ethiprole on detoxification enzyme and acetylcholin esterase activity in Frankliniella occidentalis (Pergande)
李定银 郅军锐 张 涛 曾 广
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DOI：10.7679/j.issn.2095-1353.2020.143
作者单位：贵州大学昆虫研究所，贵州省山地农业病虫害重点实验室，贵阳 550025；贵州大学昆虫研究所，贵州省山地农业病虫害重点实验室，贵阳 550025；贵州大学昆虫研究所，贵州省山地农业病虫害重点实验室，贵阳 550025；贵州大学昆虫研究所，贵州省山地农业病虫害重点实验室，贵阳 550025
中文关键词：西花蓟马；乙基多杀菌素；乙虫腈；亚致死浓度；解毒酶
英文关键词：Frankliniella occidentalis; spinetoram; ethiprole; sublethal concentration; detoxification enzyme
中文摘要：
【目的】 为探讨乙基多杀菌素（Spinetoram）和乙虫腈（Ethiprole）对西花蓟马Frankliniella occidentalis的毒力，及对解毒酶及乙酰胆碱酯酶活性影响。【方法】 采用浸叶法分别测定了2种杀虫剂对西花蓟马的毒力，以及亚致死浓度（LC25）分别处理西花蓟马2龄初期若虫12、24、48和72 h后虫体内羧酸酯酶（CarE）、谷胱甘肽-S-转移酶（GSTs）、微粒体多功能氧化酶（MFOs）、细胞色素P450单加氧化酶（P450）和乙酰胆碱酯酶（AChE）活性的变化。【结果】 西花蓟马对乙虫腈和乙基多杀菌素均较为敏感，亚致死浓度（LC25）分别为0.708 9 mg/L和0.003 3 mg/L。亚致死浓度乙基多杀菌素处理下，西花蓟马体内CarE、GSTs、MFOs和P450活性不论在何时间下均显著升高，AChE活性在12-48 h均受到抑制，在72 h和对照没有显著差异。乙虫腈处理下西花蓟马体内CarE和P450活性显著升高，GSTs活性和对照无明显变化，MFOs活性在在12-24 h 和对照没有明显差异，在48 h和72 h后显著高于对照，AchE活性显著降低。【结论】 乙基多杀菌素和乙虫腈亚致死浓度处理下，西花蓟马体内的解毒酶和乙酰胆碱酯酶活性发生不同变化，响应的程度与药剂的种类有关，且具有时间效应。
英文摘要：
[Objectives] To determine the toxicity of spinetoram and ethiprole to Frankliniella occidentalis, and the effect of these pesticides on detoxification enzymes and acetylcholinesterase activity. [Methods] The effects of sublethal concentrations (LC25) of spinetoram and ethiprole on 2nd instar nymphs of F. occidentalis were measured using the leaf dipping method. Changes in carboxylesterase (CarE), glutathione S-transferase (GSTs), cytochromeP450 (P450), multi-function oxidase (MFO) and acetylcholin esterase (AChE) activity were measured 12, 24, 48 and 72 hours, after insects had been treated with LC25 doses of spinetoram and ethiprole. [Results] F. occidentalis was sensitive to both spinetoram and ethiprole and the sublethal LC25 concentrations of these pesticides were 0.7089 mg/L and 0.0033 mg/L, respectively. CarE, GSTs, MFOs and P450 activity increased significantly after treatment with an LC25 dose of spinetoram. AChE activity decreased from 12 to 48 hours after treatment, falling to the level of the control group by 72 hours after treatment. After ethiprole treatment, CarE and P450 activity increased significantly but GSTs activity didn’t change significantly, compared to the control. MFOs activity didn’t differ significantly from that of the control after 12 and 24 hours, but was higher than the control 48 and 72 hours after treatment. AChE decreased significantly throughout the experimental period. [Conclusion] Sublethal concentrations of spinetoram and ethiprole affected the activities of detoxification enzymes and acetylcholin esterase in F. occidentalis to different degrees after different periods of time post-treatment.