四川省二化螟不同地理种群遗传多态性的差异分析
Genetic diversity of geographical populations of Chilo suppressalis (Walker) in Sichuan province
于文娟；陈 丹；罗 涵；姬红丽；彭云良
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DOI：10.7679/j.issn.2095-1353.2021.013
作者单位：1. 四川省农业科学院植物保护研究所，农业部西南作物有害生物综合治理重点实验室，成都 610066； 2. 中国水稻研究所，水稻生物学国家重点实验室，杭州 311401
中文关键词：二化螟；遗传多样性；COⅡ；ITS；地理种群
英文关键词：Chilo suppressalis (Walker); genetic diversity; cytochrome C oxidase subunit Ⅱ; internal transcribed spacer; geographical population
中文摘要：
【目的】 为了解四川省二化螟Chilo suppressalis (Walker) 不同地理种群的遗传结构，分析各个种群间的亲缘关系，为区域治理对策提供新的依据。【方法】 通过测定四川省17个市县二化螟样本的线粒体COⅡ基因和核糖体ITS基因，利用MEGA软件分析二化螟不同地理种群的基因遗传多样性，以获取样本群体遗传多样性信息。【结果】 对114条幼虫线粒体COⅡ基因序列分析发现，在500 bp的区段中共有147个位点存在多态性，包含33个单倍型；川东地区和川西地区二化螟种群的单倍型多样度分别为0.703 83和0.802 26；系统发育树显示，除眉山-1-1种群之外，其他二化螟地理种群聚合为一个大分支。而对同批二化螟核糖体ITS基因序列分析结果表明，在533 bp的区段中存在299个位点多态性，有92个单倍型；川东地区和川西地区二化螟种群的单倍型多样度分别为0.967 48和0.975 71；在系统发育树中，犍为种群聚合为一支，其余种群聚合为一支。【结论】 川西地区二化螟线粒体COⅡ基因和核糖体ITS基因多态性比川东地区样本丰富。地理种群之间的遗传分化与分布之间的相关性不大，这可能与种群本身差异相差不大相关。
英文摘要：
[Objectives] To investigate genetic diversity among geographic populations of Chilo suppressalis in Sichuan province and thereby provide information to improve the regional management of this pest. [Methods] The mitochondrial DNA (mtDNA) cytochrome oxidase subunit Ⅱ (COⅡ) and internal transcribed spacers (ITS) located in ribosomal DNA (rDNA) of 17 geographical Sichuan populations of C. suppressalis were sequenced and compared with MEGA. [Results] A 500 bp segment of the COⅡ gene among 114 mature larvae was sequenced and analyzed, and 147 variable sites and 33 haplotypes identified. The haplotype diversity of different populations ranged from 0.703 83 in eastern Sichuan to 0.802 26 in western Sichuan. Phylogenetic trees showed that all geographic populations except Meishan-1-1grouped in a single great clade. Sequencing of a 533 bp ITS segment identified 299 variable sites and 92 haplotypes. Haplotype diversity among the different populations ranged from 0.967 48 in eastern Sichuan to 0.975 71 in western Sichuan. Phylogenetic trees indicate that the Qianwei population groups in a single clade whereas all other geographic populations are in another clade. [Conclusion] Both COⅡ and ITS haplotype diversity indicate that C. suppressalis are more diverse in western Sichuan than other parts of the province. There is little correlation between genetic diversity and geographic distribution, which may reflect the fact that there was originally low divergence among populations.