[Objectives] To investigate the activity of several detoxifying enzymes, protective enzymes and metabolic enzymes in the aphid
Acyrthosiphon solani (Kaltenbach) induced by deltamethrin and high temperature stress, and thereby provide a theoretical basis for further research on pesticide resistance and predicting the occurrence of this pest
.【
Methods】Third instar nymphs of
A. solani were treated with an LC
50 dose of deltamethrin and exposed to 32 ℃ for 6, 12, 24, 48 and 72 h, after which the activity per unit mass of glutathione s-transferase, peroxidase, carboxylesterase and trehalase were measured and compared. 【
Results】 Peroxidase and carboxylesterase activity were strongly affected by both deltamethrin and high temperature, the activity per unit mass of peroxidase was significantly inhibited by deltamethrin 6, 24 and 48 h
(
P<0.05) after treatment and was higher than the control in all temperature treatment groups, significantly so in the 6, 12 and 24 h treatment groups (
P<0.05). Carboxylesterase activity was significantly different to the control 6, 24, 48 and 72 h after deltamethrin treatment (
P<0.05). Trehalase activity was significantly higher than the control 6, 24, 48 and 72 h after deltamethrin treatment and after 6, 12 and 72 h exposure to 32 ℃ (
P<0.05). Deltamethrin and temperature had relatively little effect on glutathione s-transferase activity which was only significantly different to the control 6 h and 24 h after deltamethrin treatment, and after 24 h exposure to 32 ℃ (
P<0.05).【
Conclusion】 An LC
50 dose of deltamethrin had a greater effect on the enzyme activity of
A. solani than exposure to 32 ℃; glutathione s-transferase, peroxidase, carboxylesterase and trehalase activity were all affected by both deltamethrin and temperature, but peroxidase and carboxylesterase were the most significantly affected.