六星黑点豹蠹蛾性信息素活性成分的提取和初步鉴定
The extraction and identification of active components of the sex pheromones of Zeuzera leuconotum Butler (Lepidoptera: Cossidae)
刘红霞；荆小院；郑海霞；杨美红；张金桐；朱卫华；刘金龙
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DOI：10.7679/j.issn.2095-1353.2021.047
作者单位：山西农业大学（山西省农业科学院）基础部，太谷 030801；山西农业大学（山西省农业科学院）生命科学学院，太谷 030801； 山西农业大学（山西省农业科学院）农学院，太谷 030801；山西省怀仁市农业技术推广中心，怀仁 035300
中文关键词：六星黑点豹蠹蛾；性信息素；鉴定；触角电位
英文关键词：Zeuzera leuconotum Butler; sex pheromone; identification; EAG
中文摘要：
【目的】 初步鉴定六星黑点豹蠹蛾Zeuzera leuconotum Butler（Lepidoptera，Cossidae）雌蛾性信息素活性成分。【方法】 采用正己烷溶剂浸提法提取六星黑点豹蠹蛾性信息素腺体中的化学组分，利用毛细管气相色谱（GC）和气相色谱-质谱联用（GC-MS）技术分析性腺提取物，并运用触角电位技术（EAG）测定提取物及标准化合物的电生理活性。【结果】 GC结果显示六星黑点豹蠹蛾性腺体提取物中主要存在7种成分，其中G组分在加入标准化合物反-2-顺-13-十八碳烯醇-1-乙酸酯（E2,Z13-18:Ac）后，峰高和峰面积均相应增加。GC-MS分析结果证明G组分为E2,Z13-18:A。EAG结果显示7种组分均能引起雄蛾触角电位反应，其中对E2Z13-18:Ac的反应值最大。【结论】 本研究初步鉴定出E2,Z13–18:Ac是六星黑点豹蠹蛾的性信息主要组分，为使用性信息素防治六星黑点豹蠹蛾奠定了基础。
英文摘要：
[Objectives] To identify the
active components of the sex pheromone of female Zeuzera leuconotum Butler moths. [Methods] Sex pheromones were extracted from the sex
glands of female moths with n-hexane and the active components analyzed using
gas chromatography (GC) and gas chromatography–mass spectrometry (GC-MS).
Electroantennograms (EAG) were used to examine the EAG responses of male
antennae to extracts and standard components. [Results] GC results indicate that there are 7 chemicals
in the extract, and that the peak height and area of component G increased
after the addition of standard compound E2,Z13-18:Ac. Based on GC-MS data,
component G was identified as E2,Z13-18:Ac. The EAG results show that all
compounds caused an antennal reaction, and that E2,Z13-18:Ac elicited the
strongest EAG activity. [Conclusion] E2,Z13-18:Ac was identified as the main
component of the sex pheromone of Z. leuconotum. These results provide a theoretical basis for
developing synthetic sex pheromone lures to control this pest.