【目的】 为建立一种适用于鳞翅目昆虫类型Ⅱ性信息素环氧二烯同分异构体的分离制备方法。【方法】  本研究采用半制备高效液相色谱正向系统，结合蒸发光散射检测器和SunFire Silica OBD制备柱，建立了分离制备鳞翅目昆虫类型Ⅱ性信息素方法，制备样品经气相色谱质谱联用仪分析并最终通过田间诱捕试验验证。【结果】 采用0.5%四氢呋喃的正己烷溶液为流动相，流速为7 mL/min，蒸发光散射检测器漂移管温度为40 ℃，在增益设为500的条件下运行，类型Ⅱ性信息素环氧二烯同分异构体的分离效果较好，分离度为2.72-3.10，制备样品单体纯度高，制备样品回收率为80.73%-98.47%；将分离制备的C₁₈环氧二烯同分异构体混合物应用于田间诱捕试验，结果表明本方法制备的顺3,顺9-6,7-环氧十八碳二烯单体结合顺3,顺6,顺9-十八碳三烯可以专一性引诱灰茶尺蠖 Ectropis grisescens，28 d共计诱杀45.33头/诱捕器；制备的顺3,顺6-9,10-环氧十八碳二烯单体结合顺3,顺6,顺9-十八碳三烯可以专一性引诱优美苔蛾Miltochrista striata，28 d共计诱杀44.00头/诱捕器。【结论】 半制备高效液相色谱法是一种简单便利，高效易行的类型Ⅱ性信息素环氧二烯同分异构体的分离制备方法，可满足实验研究与小面积田间应用的需求。

[Objectives]  To establish an effective method for the separation and preparation of diene monoepoxides regioisomers mixtures of the Lepidopteran Type Ⅱ sex pheromone. [Methods]  The parameters of the method were established using forward system, semi-preparative, high performance, liquid chromatography combined with an evaporative light scattering detector and SunFire Silica OBD preparative column, after which the prepared monomers were verified by gas chromatography-mass spectrometry and field trapping. [Results]  The semi-preparative high performance liquid chromatography was eluted with 0.5 % tetrahydrofuran in hexane at a flow rate of 7.0 mL/min with the drift tube temperature of the evaporative light scattering detector set at 40 ℃ and the gain value set at 500. This method efficiently separated diene monoepoxides regioisomers mixtures of the Type Ⅱ sex pheromone with a resolution of 2.72-3.10. The purity of the prepared compounds was significant and the recovery rate of prepared samples was 80.73%-98.47%. Field trapping tests confirmed that the prepared Z3, epo6 and Z9-18:H, in combination with Z3, Z6 and Z9-18:H, specifically attracted Ectropis grisescens (45.33 moths/trap over 28 d), whereas the prepared Z3, Z6 and epo9-18:H, in combination with Z3, Z6 and Z9-18:H, specifically attracted Miltochrista striata (44.00 moths/trap over 28 d). [Conclusion]  Semi-preparative high performance liquid chromatography is a simple, convenient and efficient method for the separation and preparation of diene monoepoxides regioisomers mixtures of the Lepidopteran TypeⅡ sex pheromone, and prepared samples can be used for sex pheromone studies and small-scale field applications.