雷帕霉素对二种鳞翅目昆虫细胞自噬和凋亡的影响
Influence of rapamycin on autophagy and apoptosis of two lepidopteran cell lines
李佳芮，邓玉杰，张许平，付 涛，马 源，郭建军，刘凯于
点击：2336次 下载：0次
DOI：
作者单位：华中师范大学 生命科学学院
中文关键词：自噬作用；细胞凋亡；斜纹夜蛾细胞系（Sl-HP），BMN-e细胞
英文关键词：autophagy, apoptosis, Spodoptera lituracell line (SL-HP), BMN-e cells
中文摘要：以2种鳞翅目昆虫细胞为材料，采用雷帕霉素进行处理，初步研究自噬作用与昆虫细胞凋亡的关系。结果表明：雷帕霉素能够提高家蚕细胞系BMN-e细胞的自噬水平，并能诱导BMN-e细胞发生凋亡；自噬抑制剂3-甲基腺嘌呤能抑制雷帕霉素诱导的BMN-e细胞凋亡。相反，雷帕霉素虽能诱导斜纹夜蛾细胞系SL-HP细胞的自噬水平提高，但不能诱导斜纹夜蛾Spodoptera litura(Fabricius)细胞发生凋亡；雷帕霉素的预处理能抑制放线菌素D 诱导的斜纹夜蛾细胞系SL-HP细胞发生凋亡；自噬抑制剂3-甲基腺嘌呤对放线菌素D诱导的细胞凋亡没有影响。因此家蚕Bombyx mori细胞自噬水平的提高与细胞凋亡具有正相关性，而斜纹夜蛾细胞自噬水平的提高与细胞凋亡不相关，相反还对细胞凋亡的诱导具有一定的抑制作用。
英文摘要：In order to understand the relationship between autophagy and apoptosis, we investigated autophagy and apoptosis in two Lepidopteran cell lines, BMN-e cell, and Spodoptera litura(Fabricius) cell line (SL-HP) after treatment with rapamycin. Rapamycin induced autophagy and apoptosis in BMN-e cells, while 3-methyladenine suppressed autophagy and apoptosis induced by rapamycin in BMN-e cells. However, rapamycin induced autophagy but did not induce apoptosis in SL-HP cells. When the cells were treated with rapamycin in advance, apoptosis induced by actinomycin D was partially inhibited, but 3-methyladenine had no effect on apoptosis induced by actinomycin D. It indicated that autophagy and apoptosis in BMN-e cells had a positive correlation. In contrast, there was no correlation in SL-HP cells, and the increasing of autophagy inhibited apoptosis in this cell line in some extent.