蜜蜂微孢子虫在中国的自然种系构成初探
Natural stripe of Microsporidia of honeybee in China
刘 锋,王 强,代平礼,吴艳艳,宋怀磊,周 婷**
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DOI：
作者单位：中国农科院蜜蜂研究所 农业部授粉昆虫生物学重点开放实验室 北京 100093
中文关键词：蜜蜂微孢子虫;西方蜜蜂微孢子虫; 东方蜜蜂微孢子虫; 16s rRNA基因
英文关键词：Microsporidia, Nosema apis, Nosema ceranae, 16s rRNA gene
中文摘要：蜜蜂微孢子虫 (Nosema apis及 Nosema ceranae)是微孢子虫的典型代表之一，由它寄生蜜蜂所产生的疾病，称为蜜蜂微孢子虫病。通过目前国际上普遍使用的克隆16s rRNA基因（16s rDNA） 的片段并测序的方法来进行蜜蜂微孢子虫在我国主要养蜂地区的分布情况。结果表明，在我国主要养蜂地区，造成大量西方蜜蜂患蜜蜂微孢子虫病的是东方蜜蜂微孢子虫Nosema ceranae，至今为止未发现过去我们广泛认定的西方蜜蜂微孢子虫Nosema apis。
英文摘要：
Microsporidia is a kind of protist that parasitizes in the cell. Nosema apis and Nosema ceranae are two typical types of them. They cause the Nosema disease. This article mainly introduces the distribution of the two Microsporidia spp. in China by cloning and sequencing of the segment of 16s rDNA. The results showed that Nosema ceranae was the agent of cause Nosema disease of Apis melliffera in most provinces of China. Nosema apis that was considered as the pathogen of Nosema disease in China has not been found until now.