麦长管蚜ELISA检测体系的建立
高书晶1，2；庞保平1**；陈正贤3
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DOI：
作者单位：1内蒙古农业大学农学院 呼和浩特 010019； 2中国农业科学院草原研究所 呼和浩特 010010；3浙江大学生物技术研究所 杭州 310029
中文关键词：麦长管蚜， 单克隆抗体， 酶联免疫吸附试验， 灵敏度
英文关键词：Macrosiphum avenae, monoclonal antibody, ELISA, sensitivit
中文摘要：应用麦长管蚜Macrosiphum avenae(Fabricius)单克隆抗体比较直接、间接和夹心ELISA方法。结果表明，间接ELISA的灵敏度高于直接ELISA，灵敏度分别为0.042 μg/mL和0.083 μg/mL，而双抗夹心ELISA灵敏度很差，无法使用。建立用于检测捕食性瓢虫肠道内麦长管蚜抗原的间接ELISA检测体系，为进一步应用单克隆抗体评价捕食性瓢虫对麦长管蚜的控制作用奠定可靠的基础。
英文摘要：Direct, indirect and double-antibody-sandwich enzymelinked immunosorbent assay (ELISA) were examined with the monoclonal antibodies to detect the antigen of Macrosiphum avenae (Fabricius) in the gut of predatory ladybirds. The results showed indirect ELISA was more sensitively than direct ELISA, in which the detection sensitivities were 0.042 μg/mL and 0.083 μg/mL, respectively wheres double antibody sandwich ELISA was not sensitively to be used. An indirect ELISA method was set up in order to detect the antigen of M. avenae in the gut of predatory ladybirds, which established a reliable foundation for evaluating the predation of predatory ladybirds on M. avenae with the monoclonal antibodies.