微生态制剂EM原露对熊蜂蜂王产卵前期卵巢发育及卵子发生的影响
The effect of effective microorganisms on the ovarian development and oogenesis of bumblebee queens in preoviposition period
廖思米1，2;周祖基1**;耿金虎2***;徐希莲2 ;王凤鹤2
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作者单位：1四川农业大学森林保护省级重点实验室 四川雅安 625014； 2北京市农林科学院农业科技信息研究所 北京 100097
中文关键词：微生态制剂EM原露，熊蜂，卵巢发育，卵子发生
英文关键词：effective microorganisms(EM), bumblebee (Bombus terrestris), ovarian development, oogenesis
中文摘要：用60%蔗糖，60%蔗糖加EM原露，60%蔗糖加花粉，60%蔗糖加花粉加EM原露4种食料饲喂复苏熊蜂Bombus terrestris L.蜂王，研究EM原露对复苏蜂王产卵前期卵巢发育及卵子发生的影响。结果表明，EM原露对熊蜂蜂王产卵前期卵巢发育及卵子发生表现出一定抑制性，但影响不显著。虽然于第2天，EM原露对其开始发生显著抑制性影响(P=0.047)，且EM原露与花粉的交互作用对其发育促进影响亦显著(P=0.014)，但从第5天始，EM原露对其影响下降为不显著，而花粉开始表现出显著性促进熊蜂蜂王卵巢发育(P=0.000)。EM原露对熊蜂蜂王卵子发生并无促进或抑制作用，然而花粉的缺失促使熊蜂蜂王卵子发生停滞。
英文摘要：The effects of effective microorganisms(EM) on the ovarian development and oogenesis of bumblebee queens in preoviposition period were assessed by feeding the recoverial bumblebee queens on 60% sugar, 60% sugar+EM, 60% sugar+pollen, 60% sugar+pollen+EM. The result showed that the EM is not able to statistically affect the ovarian development and oogenesis of bumblebee queens in preoviposition period. Despite the EM alone or interactions between the EM and pollen inhibited ovarial development significantly in 2 days (P=0.047, P=0.014), the effects of pollen promoted significantly ovarian development (P=0.000), but EM did not from the 5th day to the 9th day, The oogenesis of bumblebee queen wasn't affected by EM significantly, and the ovarian development level was forced to inhibit by lack of pollen.