基于18S rRNA和线粒体16S rRNA基因序列的柳蚕进化分析
Phylogenetic analysis of Actias selene based on 18S ribosomal RNA and mitochondrial 16S rRNA genes
朱保建，刘朝良**，曹甲，李旭全，魏国清
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作者单位：安徽农业大学生命科学学院合肥230036
中文关键词：柳蚕,核糖体18S RNA,线粒体16S rRNA,进化分析
英文关键词：Actias selene, 18S ribosomal RNA, mitochondrial 16S rRNA, phylogenetic relationship
中文摘要：
为探讨柳蚕Actias selene Hübner与鳞翅目昆虫的系统发育关系，本研究利用PCR扩增获得了柳蚕核糖体18S rRNA和线粒体16S rRNA基因的部分序列，长度分别为391 bp 和428 bp。并采用邻近距离法（NJ)、最大简约法（MP）、类平均聚类法（UPGMA）构建系统进化树。结果表明，柳蚕线粒体16S rRNA基因序列与大蚕蛾科昆虫的16S rRNA基因序列均表现出偏好于碱基AT的倾向。柳蚕与所研究的其它蚕类的遗传距离介于0.016至0.140之间，其中与温带柞蚕Antheraea roylii的遗传距离最小，与野桑蚕Bombyx mandarina的遗传距离最大。而基于鳞翅目昆虫18S rRNA基因部分序列的进化分析显示，柳蚕与柞蚕Antheraea pernyi之间的遗传距离最小（0.010），与蓖麻蚕Samia ricini的遗传距离最大（0.017）。
英文摘要： To investigate the phylogenetic relationship between Actias selene Hübner and other Lepidopteran insects, a 391 bp fragment of 18S ribosomal RNA and a 428 bp fragment of mitochondrial 16S rRNA were used to construct phylogenetic trees with neighbor\|joining (NJ), maximum parsimony (MP) and arithmetic averages (UPGMA) methods. The results showed the nucleotide composition of mitochondrial 16S rRNA gene of A. selene had AT bias, similar in other Saturnidae insects. The genetic distances between A. selene and other species ranged from 0.016 to 0.140 based on mitochondrial 16S rRNA gene sequences, in which the minimum distance was that to Antheraea roylii and the maximum was that to Bombyx mandarina. As far as 18S rRNA gene sequences were concerned, the minimum distance (0.010) and the maximum distance (0.017) were showed between A. selene with A. pernyi, and Samia ricini, respectively.