一种经济高效提取实蝇基因组DNA的方法
An effective and economical method for extracting genomic DNA from the fruit fly
杨 燕1， 白永华2 ， 王绍君2，周 剑2 ，李克海2 ，丁元明2**
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DOI：
作者单位：1. 云南农业大学 云南 650201； 2. 云南出入境检验检疫局 云南 650228
中文关键词：
英文关键词：genomic DNA, fruit fly,extraction
中文摘要： 单头实蝇高质量基因组DNA的获得为实蝇分子生态学研究奠定了重要基础。本文提出一种经济高效的实蝇基因组DNA提取方法，该方法广泛适用于不同虫态、不同保存条件的实蝇标本，与传统的CTAB法和SDS法相比操作简单、耗时短，得率高。
关键词 基因组DNA, 实蝇, 提取
英文摘要： An effective method for extracting genomic DNA from individual specimens is important for the study of molecular phylogenitics in the fruit fly. This paper presented a method for extracting genomic DNA from individual fruit fly.The results showed that the genomic DNA could be successfully extracted from adult, larval and pupal of fruit flies. Meanwhile, this method is also suitable for dried specimens or specimens preserved in alcohol. Compared with traditional methods like CTAB and SDS, it is pretty simple, time saving and effective.