黑水虻幼虫肠道和体表产酶细菌的分离和鉴定
Isolation and identification of bacteria producing enzymes from gut and skin of black soldier fly（Hermetia illulens）larvae.
喻国辉1,牛春艳1，2,何国宝1，2,周 林1，3,夏 嫱4,程 萍1**
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DOI：
作者单位：1.珠海市农业科学研究中心 珠海 519075；2.中山大学生命科学学院 广州 510275；3.华中农业大学生命科学学院 武汉 430070；4.遵义医学院珠海校区 珠海 519401
中文关键词：黑水虻, 共生微生物, 蛋白质分解, 有机磷分解, 枯草芽孢杆菌
英文关键词： black soldier fly, companion microbe, protein catabolism, organic phosphate catabolism, Bacillus subtilis
中文摘要： 从野外收集和室内饲养的黑水虻Hermetia illucens L.幼虫体表和肠道分离出同时具蛋白质和有机磷分解能力的细菌10株，其中编号为T2、T4、T6、T7和T8的菌株分离自体表，编号为S14、S15、S16、S19和S20的菌株分离自肠道。克隆细菌的16S rDNA和DNA促旋酶B亚基编码基因gyrB对10株细菌进行了鉴定。通过16S rDNA序列确定10株菌为芽孢杆菌属，根据gyrB基因以及BLAST结果构建系统发育树，将10株细菌鉴定为枯草芽孢杆菌。然后将10株菌的gyrB基因以B. subtilis subsp. subtilis str.168和模式菌株B. subtilis subsp. subtilis BCRC10255相应基因序列为参照构建系统发育树，分析10株菌在种的水平上的亲缘关系，发现10株菌存在亲缘关系差异，没有重复菌株。
关键词
英文摘要： Ten bacterial strains were isolated from the gut, or skin, of the black soldier fly (Hermetia illucens L.) larvae, collected from the field or when feeding indoors. The strains from the gut were named S14, S15, S16, S19 and S20, and those from skin were named T2, T4, T6, T7 and T8. All strains had both protein and organic phosphate metabolic capability. The 16S rDNA sequences of these strains were cloned; BLAST analytical results identified all strains as Bacillus species. The gyrB gene, which encodes DNA gyrase B protein, was used to identify the strains to the species level; all strains were identified as B. subtilis. A phylogenetic tree based on the gyrB gene sequences of the ten strains, B. subtilis subsp. subtilis str.168 and strain BCRC10255, indicates that the ten B. strains have phylogenetic relationships and that each is unique.