寄主胁迫下小菜蛾种群的RAPD分析
Analysis of diamondback moth populations under hostplants stress by RAPD
罗德，黄斌，侯有明**
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作者单位：福建农林大学植物保护学院 农业部亚热带农业生物灾害与治理重点开放实验室福州350002
中文关键词：小菜蛾，寄主胁迫，种群变异，RAPD
英文关键词： Plutella xylostella, host plants stress, population variation, RAPD
中文摘要： 采用RAPD-PCR技术，研究了取食6种不同十字花科寄主植物第4、9、15代小菜蛾Plutella xylostella (L.)种群的DNA多态性。通过17条引物的重复PCR扩增实验，利用NTsys软件对电泳条带统计分析，构建了遗传距离矩阵和聚类分析图谱。结果表明：取食芥蓝的小菜蛾种群与其它5种寄主种群遗传距离较远（0.6523～0.8246），独立聚为一枝；取食萝卜的小菜蛾种群遗传距离与甘蓝上种群较近（0.3443），聚为一枝，并且与白菜、芥菜和菜心上小菜蛾种群遗传距离较近，逐次聚为一枝，芥蓝、萝卜及甘蓝寄主种群随代次的增多种群分化出现稳定规律性。各寄主种群间遗传距离平均值和范围、多态性条带占总条带数目的比例随代次增多而增大，说明种群分化程度随代次增多而加深。
英文摘要： DNA polymorphism of the fourth, ninth, and fifteen generations’ of diamondback moth (DBM) Plutella xylotella (L.) populations, feeding on six different cruciferous host plants, was studied using the RAPDPCR technique. After repeated DNA amplification with 17 primers, electrophoretic bands were statistically analyzed with NTsys software and a genetic distance matrix and dendrogram constructed. The results indicate that the population feeding on Brassica alboglabra Bailey clustered independently on one branch and was separated from the other five populations by the largest genetic distance (0.6523-0.8246). The populations feeding on Raphanus sativus L. and B. oleracea L. clustered on one branch and the genetic distance between these was small（0.3443）. The genetic distance between populations feeding on B. chinensis L., B. juncea L. and B. parachinensis Bailey was also small and these populations also clustered on a single branch. With increasing generations the genetic differentiation of populations feeding on B.juncea L., R. sativus L. and B. oleracea L stabilized. As the generations increased, the average value, range of genetic distance between each host population and the proportion of polymorphic bands in the total number of bands enlarged. This shows that the level of population differentiation increases with generation.