小菜蛾高效Bt菌株的分离、生化特性及基因型鉴定
Isolation, biochemical and crytype gene characterization of Bacillus thuringiensis strains with high toxic to Plutella xylostella
朱勋1,2，吴青君2，张友军2，刘春光1，刘岩林1，余亚军1，景伟1，薛原1，杨峰山1*
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作者单位：1黑龙江大学生命科学学院 微生物黑龙江省高校重点实验室哈尔滨150080；2中国农业科学院蔬菜花卉研究所北京100081
中文关键词：苏云金芽孢杆菌，鉴定，杀虫活性，cry/cyt基因
英文关键词： Bacillus thurigiensis, identification, insecticidal activity, cry/cyt genes
中文摘要： 从哈尔滨田间采集死亡小菜蛾Plutella xylostella (L.)幼虫，从中分离出10株苏云金芽孢杆菌Bacillus thuringiensis。对小菜蛾幼虫室内生物测定结果表明，各菌株对小菜蛾幼虫的死亡率均在85%以上，其中DBW902毒力最强，48 h的LC50为13.99 mg/L。菌株cry/cyt基因检测表明，所有菌株均含有cry1A或cry2A基因，这与其高毒力的特性基本吻合。生化检测与分析表明，菌株DBW904、DBW93、DBW962与已报道的苏云金芽孢杆菌山东亚种B.thuringiensis subsp.shandongiensis的生化特性一致，但是其它菌株的生理生化特性与已报道菌株有区别。
英文摘要： The insecticidal activities of ten strains of Bacillus thuringiensis isolated from diseased Plutella xylostella (L.) larvae were characterized. All strains caused >80% larval mortality and the strain DBW902 had the highest insecticidal activity with an LC50 of 13.99 mg/L at 48 h. The biochemical characteristics and cry/cyt genes of each strain were determined. High insecticidal activity coincided with the detection of at least one cry1A or cry2A family gene. The biochemical characteristics of the strains, DBW904, DBW93 and DBW962 were homologous to B. thuringiensis subsp. and Shandongiensis, but not the other strains. The identification of these 10 B. thuringiensis isolates with moderate to high insecticidal activity against P. xylostella is promising for the control of this pest in northeastern China.