家蚕para钠通道cDNA片段克隆与序列分析
Cloning and sequence analysis of para sodium channel cDNA fragment from silkworm, Bombyx mori
何琳*,刘丽花,汪洋
点击：1849次 下载：1次
DOI：
作者单位：华东师范大学生命科学学院上海200062
中文关键词：家蚕，para型钠离子通道，基因克隆，序列分析
英文关键词：silkworm，para-sodium channel，gene clone，sequence analysis
中文摘要： 昆虫神经系统para型钠离子通道是拟除虫菊酯类杀虫剂的主要靶标，已有的研究表明钠离子通道基因发生点突变与昆虫对菊酯类杀虫剂的抗性密切相关。本文通过RT-PCR方法克隆获得了编码家蚕Bombyx mori L.钠离子通道的cDNA片段（GenBank No.EF521818），该片段全长4 882 bp，部分ORF包含3 986 bp核苷酸，翻译成1 328个氨基酸。蛋白序列分析表明，PCR扩增获得的家蚕钠离子通道cDNA片段所编码的氨基酸与其他昆虫的para型钠离子通道α亚基的氨基酸具有很高的同源相似性，与棉铃虫Heliothis virescens Fabricius、埃及伊蚊Aedes aegypti L.、德国小蠊Blattella germanica L.、果蝇Drosophila melanogaster Meigen和家蝇Musca domestica L.的相似性分别为95%、82%、80%、79%、77%。
英文摘要：Previous studies have revealed that a point mutation of a target gene is related to insecticide resistance to pyrethroids.The para sodium channel in the insect central nervous system is the target of pyrethroid insecticides.We used the RT-PCR method to clone the para sodium ion channel in the silkworm, Bombyx mori L.（GenBank No.EF521818）.The full length of this cDNA fragment is 4 882 base pairs and its partial ORF is 3 986 bp translated into 1 328 amino acids.BLAST analysis demonstrated that the cloned cDNA fragment is virtually identical to the para sodium channel α subunit gene amplified from other insects.Amino acid homology of the cloned fragment with para sodium channel α subunit genes from Heliothis virescens Fabricius, Aedes aegypti L., Blattella germanica L., Drosophila melanogaster Meigen and Musca domestica L.was 95%, 82%, 80%, 79% and 77% respectively.