亚致死剂量杀虫剂对意蜂工蜂嗅觉敏感性的影响
Effects of sublethal doses of insecticides on the olfactory sensitivity of the honeybee（Apis mellifera ligustica）
宋怀磊**，周婷***，王强，代平礼，罗其花，徐书法，吴艳艳
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作者单位：中国农业科学院蜜蜂研究所 农业部授粉昆虫生物学重点开放实验室北京100093
中文关键词：蜜蜂，溴氰菊酯，吡虫啉，喙伸反应，嗅觉，亚致死剂量
英文关键词：Apis mellifera ligustica, deltamethrin, imidacloprid, proboscis extension reflex (PER), olfactory sensation, sublethal dose
中文摘要： 喙伸反应（PER）试验适用于评价杀虫剂对蜜蜂行为的影响。本实验利用喙伸反应研究了亚致死剂量（LD50/100~LD50/10）溴氰菊酯和吡虫啉对意蜂Apis mellifera ligustica L.工蜂嗅觉敏感性的影响。结果发现，经口饲喂溴氰菊酯5 ng和10 ng后，工蜂对0.1%的蔗糖溶液的敏感性显著下降（P<0.05），水应激指数降低，但对0.3%，1%，3%，10%和30%的蔗糖溶液的敏感性没有显著变化；而经口饲喂吡虫啉0.3 ng和0.6 ng后，工蜂对上述各浓度蔗糖溶液的敏感性变化不明显，但其水应激指数升高。
英文摘要： Previous studies have shown that observations of the proboscis extension reflex (PER) are of interest for assessing the behavioral effects of insecticides on the honeybee.In the present study, the effects of sublethal doses (LD50/100—LD50/10) of deltamethrin and imidacloprid on the olfactory sensitivity of honeybees (Apis mellifera ligustica L.) were determined under controlled laboratory conditions using a conditioned PER assay.After oral consumption, deltamethrin reduced both sensitivity to antennal stimulation by sucrose solutions and the waterinduced proboscis extension reflex at doses of 5 ng/bee and 10 ng/bee.Unlike deltamethrin, imidacloprid had no effect in olfactory sensitivity assays but increased the waterinduced proboscis extension reflex.