（1. 湖北省农业科学院粮食作物研究所武汉430064； 2. 武汉市东西湖区农科所武汉430040）摘要褐飞虱Nilaparvata lugens (Stl)是危害水稻的主要虫害之一，发掘和利用新的抗褐飞虱基因培育抗性品种是目前防治褐飞虱最经济有效的方法之一。抗褐飞虱基因来自药用野生稻的抗虫品种B5，对褐飞虱生物型1和2具有高度抗性，B5携带的抗性基因Bph14被定位在第3染色体上。本研究以B5-10为抗源，以优良杂交稻亲本扬稻6号为受体亲本，通过复交和回交，利用与Bph14紧密连锁的分子标记MRG2329在后代中进行分子标记辅助选择，通过苗期分子标记检测和成株期农艺性状选择，最后育成恢复系R476和杂交组合广两优476。采用苗期群体鉴定技术对R476和广两优476的褐飞虱抗性进行了鉴定，R476和广两优476的抗性水平分别为中抗和中感。广两优476在稻飞虱发生较重的稻田进行试种示范，与对照品种扬两优6号和两优培九相比，广两优476对稻飞虱表现出明显的抗性。研究结果表明在育种过程中利用分子标记辅助选择Bph14基因是培育抗褐飞虱水稻品种的有效途径之一。
关键词水稻， 褐飞虱， 标记辅助选择， 抗性

Breeding of the brown planthopper resistant rice varieties LI JinBo1**WAN BingLiang1***XIA MingYuan1QI HuaXiong1
SHI HuaSheng2XIN FuLin2
(1. Food Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan430064, China;
2. Dongxihu District Agricultural Sciences Research Institute, Wuhan430040, China )

AbstractNilaparvata lugens (Stl) (BPH) is one of the major pests of cultivated rice. One effective method of BPH control is to identify new genes for BPH resistance and breed resistant cultivars incorporating these genes. B5 is a rice line derived from wild rice Oryza officinalis that is highly resistant to the BPH biotypes 1 and 2. The dominant BPH resistance gene in B5, Bph14, has been identified and mapped on rice chromosome 3. In this study, B510 was used as a BPH resistance donor and the elite restorer rice line Yangdao 6 was the recipient of the BPH resistance gene Bph14. Molecular marker MRG2329 linked with Bph14 was used to select this gene in segregating populations. We performed molecular markerassisted selection (MAS) at the seedling stage and selection for agronomic traits at the adult plant stage to obtain the hybrid rice restorer line R476 and the combination Guangliangyou 476 which carried the Bph14 gene. The BPH resistance levels of R476 and Guangliangyou 476 were determined in a mass seedling screening test. The results indicate that R476 was moderately resistant and Guangliangyou 476 was moderately susceptible. Guangliangyou 476 was, however, clearly more resistant to the rice planthopper than the control cultivars Yangliangyou 6 and Liangyoupeijiu in a field experiment. These results suggest that MAS of Bph14 can be effectively utilized in breeding programs for BPHresistant rice strains.
Key wordsrice, Nilaparvata lugens, molecular markerassisted selection (MAS), resistance