(河南农业大学生命科学学院郑州450002)摘要根据杨扇舟蛾颗粒体病毒(Clostera anachoreta Granulovirus,ClanGV)的全基因组序列,分析其基因组含有3个杆状病毒成纤维细胞生长因子(fibroblast growth factor,FGF)的同源ORFs。通过生物信息学软件及在线工具对ClanGVFGFs进行了分析,包括同源性、一级结构、二级结构和进化分析等。分析结果显示,测序得到的3个ClanGVFGFs转录方向不同,但都属于病毒转录早期基因;三者同源性相差较大;进化分析显示,ClanGVFGF3和NPV的FGFs进化关系较近,而ClanGVFGF1和ClanGVFGF2则与其它GV相应的FGF进化关系更近。
关键词杨扇舟蛾颗粒体病毒,成纤维细胞生长因子(FGF),序列分析
Sequence analysis of fgf genes of Clostera anachoreta Granulovirus LIANG ZhenPu XU FengZHANG XiaoXia**YIN XinMing
(College of Life Sciences, Henan Agricultural University, Zhengzhou450002,China)
AbstractBased on the whole genome of Clostera anachoreta Granulovirus (ClanGV), the Fibroblast Growth Factor (FGF) genes of ClanGV were analyzed using biosoftware and online tools. The Results show that three ClanGVFGFs, which are all early expression genes, had different transcription directions. These ClanGVFGFs had low sequence homologies but shared a similar functional domain common to all identified FGFs. Evolutionary analysis proves that ClanGVFGF3 has a close relationship to NPVFGFs, however, ClanGVFGF1 and ClanGVFGF2 are closer to GVFGF1 and GVFGF2 respectively.
Key wordsClostera anachoreta Granulovirus, Fibroblast Growth Factor (FGF), sequence analysis