Extraction of genomic DNA and optimization of RAPD system for Gomphocerus sibiricus
Author of the article:GE Feng-Wei1; JI Rong1; ZENG Xian-Chun1; WANG Ting1; WANG Xiao-Ping2
Author's Workplace:1. College of Life and Chemisty, Xinjiang Normal University, Urumqi 830054,China;2. Institute of Agricultural Professional Technology, Changji 831100,China
Key Words:RAPD(random amplified polymorphic DNA); genomic DNA; Gomphocerus sibiricus ;Balikun area
Abstract:By using an improved SDS method, we obtained high qualified genomic DNA of Gomphocerus sibiricus(L.). The effects of several parameters (contents of dNTP, Mg2+, Taq DNA polymerase and template DNA) on amplification were examined. The