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Issue:ISSN 2095-1353
           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2013年50 No.3

Analysis of Genetic differentiation in different populations of Adoxophyes orana based on AFLP
Author of the article:wei xiaotang
Author's Workplace:Shandong Entry-Exit Inspection and Quarantine Bureau
Key Words:Adoxophyes orana; AFLP; genetic differentiation; geographic populations
Abstract:The AFLP technique was used to analyze the genetic differentiation among 4 populations of Adoxophyes orana by using 3 pairs of primers, and 224 DNA fragments were generated while 195 of 224 DNA fragments were polymorphic. The genetic diversity analysis of the populations showed high level of genetic variation among 4 populations, and the Shannon’s genetic diversity indexes varied from 0.2137 to 0.2383. Nei genetic diversity indexes were from 0.1425 to 0.1578. Remarkable genetic differentiation indicated by genetic differentiation coefficient (Gst) and gene flow (Nm) analysis among 4 populations, was detected between the Summer fruit tortrix populations in the North and the Smaller tea tortrix populations in the South. There was a relatively strong level of gene flow and a low extent of genetic differentiation among the three Summer fruit tortrix populations in the North. The result of AMOVA analysis indicated that a high percentage of the total genetic variance (66.81%) accounted for the variations among populations. The UPGMA cluster analysis indicated that the genetic distances were closer among Shandong, Beijing and Shanxi populations, and thus the three populations were categorized into one clade, while Fujian population should be categorized into another clade. The results suggested that the genetic distance might be correlated with their host comparability but not related with their geographic distance.
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