Sequence Analysis of mtDNA Gene and Genetic Differentiation in Geographic Population of Empoasca vitis（Gothe)

Author of the article:LI Le, FU Jian-yu, XIAO Qiang

Author's Workplace：Tea Research Institute, Chinese Academy of Agricultural Sciences

Key Words：Tea tree; Empoasca vitis (Gothe);geographic population; mitochondrial 16S rRNA; genetic differentiation

Abstract：Abstract: Mitochondrial DNA 16S rRNA gene sequences were sequenced in 103 individuals of Empoasca vitis (Gothe) from 12 different geographical populations, including an outgroup species (Erythroneura sudra D.) . We compared the homologous sequences of all the individuals, and then calculated nucleotide composition. The median-joining network was constructed by using Network 4500. Through the sequential analyses, we observed that the value of A+T was about 76.8% and 56 nucleotide sites were substituted in the 536bp fragment of Empoasca vitis (Gothe). 56 haplotypes were detected in 100 individuals. Haplotype diversity index (H) and mean nucleotide diversity index (Pi) reached 0.971 and 0.006 respectively. The gene flow (Nm) among the 12 populations was 1.64. The overall Fst value was 0.026. Molecular variance (AMOVA) analysis indicated that a high proportion of the total genetic variance was attributable to variations with in populations (97.4%). The median-joining network of the 56 haplotypes did not distinguish distinctly into 12-location groupings of haplotypes and did not correspond to every geographic group of Empoasca vitis (Gothe).