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Your Position :Home->Past Journals Catalog->2013年50 No.4

PCR detection and sequence analysis of the Wolbachia wsp gene in four gall wasps
Author of the article:ZHAO Ling1ZHU DaoHong1**LIU ZhiWei2YANG XiaoHui1
Author's Workplace:1. Laboratory of Insect Behavior and Evolutionary Ecology, Central South University of Forestry and Technology, gsha410004, China; 2. Department of Biological Sciences, Eastern Illinois University61920, USA
Key Words:Wolbachia, wsp gene, PCR, phylogenetic relationship, Cynipini, Aylacini
Abstract:Wolbachia is a cytoplasmically inherited bacterium which occurs widely in the reproductive tissues of arthropods. It causes a wide range of alterations to host reproduction,including induction of cytoplasmic incompatibility (CI),parthenogenesis induction (PI),genetic male feminization and male mortality. We examined the presence of Wolbachia in four American gall wasps, Callirhytis punctate Bassett, Dryocosmus palustris Osten Sacken, Antistrophus silphii Gillette and Antistrophus sp., using polymerase chain reaction and sequence determination of the Wolbachia wsp gene. Wolbachia infection rates were found to be 60%and 36%, in C. punctate and D. palustris, respectively, but no infection was detected in the two Antistrophus species. The two wsp sequences of C. punctata and D. palustris have lengths of 564 bp and 561 bp, respectively, and were 94%similar. Further comparison with known wsp gene sequences in other cynipid wasps revealed a 100%similarity with those in C. punctata and Andricus solitarius (strain 1), Neuroterus macropterus and Synergus crassicornis and a similarity ranging between 79%-99%in the remaining species. Phylogenic reconstruction of the relationships of Wolbachia strains in Cynipidae with known wsp sequences indicated that the Wolbachia strain of Callirhytis punctata is closely related to that of A. solitarius (strain 1), N. macropterus, Biorhiza pallida, and S. crassicornis, whereas the Wolbachia strain of D. palustris is closely related to that of A. mairei. Addition of the new wsp sequences did not change Wolbachia’s established pattern of association in cynipid wasps, i.e., the Wolbachia strain of Ceroptres cerri belongs to the B group whereas that of all other species belong to the A group. Additionally, we used the sexually reproductive generation to examine the presence of Wolbachia both in C. punctate and D. palustris. The results indicate that Wolbachia does not induce parthenogenesis in these species.
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