Liquid chromatography and bioassay analysis of components in the trail pheromone of the blackwing subterranean termite Odontotermes formosanus Shiraki
Author of the article:WEN Ping1,3**JI BaoZhong1***LIU ShuWen2JI ShiLin1LIU JiaJia1
Author's Workplace：1. College of Forest Resource and Environment, Nanjing Forestry University, Nanjing210037, China; 2. Administration Bureau of Dr Sun YatSen’s Mausoleum, Nanjing210014, China;
Key Words：Odontotermes formosanus, trail pheromone, liquid chromatograph, active components
Abstract：By working at the threshold active concentration, the characteristic behavioral reactions of the blackwinged subterranean termite Odontotermes formosanus Shiraki to extracts of trail pheromone components were analyzed in trailfollowing bioassays, including the effect of different testing substrates. Based on the results, a standard bioassay method was used to test the fractions from isocratic elution (22% EtOAc/nhexane) of pheromone extract on a silica gel column. Results show that two active peaks exist in the fractions, which indicate that the trail pheromone of O. formosanus consists of at least two components.