Effect of sub-lethal doses of chlorpyrifos on cytochrome P450 enzyme activity and gene expression in Locusta migratoria
Author of the article:GUO Yan-Qiong1, 2** ZHANG Jian-Zhen1 GUO Ya-Ping1 MA En-Bo1***
Author's Workplace:1. Institute of Applied Biology, Shanxi University, Taiyuan 030006, China;2. College of Agriculture, Shanxi Agricultural University, Taigu 030801, China
Key Words:sublethal doses, chlorpyrifos, Locusta migratoria, cytochrome P450
Abstract: [Objectives] To investigate the effects of sub-lethal doses of chlorpyrifos (organophosphate) on cytochrome P450 activity in Locusta migratoria. [Methods] Three doses (LD10, LD30 and LD50) of chlorpyrifos were applied to specimens of L. migratoria and the cytochrome P450 monooxygenase activity of 7-EC-O-deethylation (ECOD) was measured and the mRNA levels of CYP409A1 and CYP408B1 were analyzed by Real-time RT-PCR after 24 h. [Results] ECOD activity decreased by 0.50-0.68-fold after chlorpyrifos exposure and mRNA expression of CYP409A1 and CYP408B1 also decreased. Conclusion] Chlorpyrifos can significantly inhibit cytochrome P450 enzyme activity by depressing the transcription of multiple cytochrome P450 genes.