Cloning and phylogenetic analysis of the tubulin genes of Nosema pernyi
Author of the article:WANG Yong1, 2** HUANG Ling2** JIANG Yi-Ren2 WEN Zhu2 YU Feng1, 2SHI Sheng-Lin2 QIN Li2***
Author's Workplace:1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China; 2. College of Bioscience and Biotechnology, Shenyang Agricultural University, Liaoning Engineering and Technology Resource Center forInsect Resource, Shenyang 110866, China
Key Words:Nosema pernyi, tubulin genes, phylogenetic analysis
Abstract:
[Objectives] The purpose of this project was to investigate tubulin gene sequences of Nosema pernyi in order to provide a foundation for further phylogenetic analysis. [Methods] Using RT-PCR, 3′ RACE technology, α, β and γ-tubulin genes of N. pernyi were cloned. Neighbour-Joining and Maximum Likelihood were used to construct a phylogenetic tree. [Results] α, β and γ-tubulin cDNA sequences of N. pernyi were submitted to NCBI (GenBank accession numbers: KF154086、KF023271、KF740389). Phylogenetic analysis shows that the microsporidia are a single group in the fungi that are most closely related to the Entomophthoromycota, and are sistergroups to the Basidiomycota, Chytridiomycota, Zygomycota, Glomeromycota and some of the Ascomycota. N. pernyi, together with other Nosema spp., belongs to one of two groups in the microsporidia. [Conclusion] α, β and γ-tubulin genes of N. pernyi can be successfully cloned and the resultant phylogenetic analysis provides a foundation for further taxonomic research on N. pernyi.