Comparison of the quality of genomic DNA extracted from adult specimens of Monochamus alternatus preserved by different methods
Author of the article:QU Liang-Jian1** WANG Li-Juan2 WANG Qing-Hua1 WANG Yu-Zhu1 ZHANG Yong-An1***
Author's Workplace：1. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China;2. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Key Words： Monochamus alternates, longicorn adult, DNA extraction, preservation methods
Abstract： [Objectives] To find which method of preserving Monochamus alternatus specimens produced the best quality genomic DNA. [Methods] Genomic DNA from Monochamus alternatus adults that had been preserved using different methods (liquid nitrogen, 100% ethanol at -20℃, 100% ethanol at room temperature and museum specimens) for more than 2 years, was extracted using the SDS-proteinase K method and the quality of the extracted genomic DNA compared. [Results] The best quality genomic DNA was obtained from specimens that had been stored in liquid nitrogen, and the next best from those that had been preserved in 100% ethanol at -20℃. [Conclusion] The best quality DNA was extracted from specimens that had been frozen in liquid nitrogen or preserved in 100% ethanol at -20℃. The DNA samples obtained from such specimens are suitable for PCR amplification and sequencing.