Application of DNA barcoding technology to the identification of Quadrastichus erythrinae Kim (Hymenoptera: Eulophidae)
Author of the article:HUANG Peng-Ying1** LIN Ling-Ling1 LI Wen-Lin2 HONG Qin-Yang2ZHANG Ling-Hua2 LIAO Fu-Rong1
Author's Workplace:1. Xiamen Entry-exit Inspection and Quarantine Bureau, Xiamen 361012, China;2. College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou 350003, China
Key Words:Quadrastichus erythrinae, ITS gene, DNA barcoding, diagnostic primers, molecular identification
Abstract: [Objectives] Quadrastichus erythrinae Kim (Hymenoptera: Eulophidae) is too small to be identified quickly and accurately by traditional morphological methods. [Methods] The complete sequences of the rDNA ITS1 and ITS2 genes were determined for Q. erythrinae. A phylogenetic tree was established on the basis of variation in 18SrDNA part sequences using MEGA Maximum Likelihood. Species-specific primers of Q. erythrinae were designed based on the rDNA ITS1 and ITS2 sequences. The results of PCR amplification of rDNA ITS1 and ITS2 indicate that these species-specific primers could reliably identify this species. [Results] The results suggest that the species-specific primers developed produce clear, unique and reproducible target DNA bands from individual adult wasps. Molecular identification of Q. erythrinae using species-specific primers from the rDNA ITS1 and ITS2 region is therefore feasible. [Conclusion] This study identified a possible avenue for the molecular identification of Q. erythrinae.