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           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2014年51 No.3

Application of DNA barcoding technology to the identification of Quadrastichus erythrinae Kim (Hymenoptera: Eulophidae)
Author of the article:HUANG Peng-Ying1** LIN Ling-Ling1 LI Wen-Lin2 HONG Qin-Yang2ZHANG Ling-Hua2 LIAO Fu-Rong1
Author's Workplace:1. Xiamen Entry-exit Inspection and Quarantine Bureau, Xiamen 361012, China;2. College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou 350003, China
Key Words:Quadrastichus erythrinae, ITS gene, DNA barcoding, diagnostic primers, molecular identification
Abstract:    [Objectives]  Quadrastichus erythrinae Kim (Hymenoptera: Eulophidae) is too small to be identified quickly and accurately by traditional morphological methods. [Methods]  The complete sequences of the rDNA ITS1 and ITS2 genes were determined for Q. erythrinae. A phylogenetic tree was established on the basis of variation in 18SrDNA part sequences using MEGA Maximum Likelihood. Species-specific primers of Q. erythrinae were designed based on the rDNA ITS1 and ITS2 sequences. The results of PCR amplification of rDNA ITS1 and ITS2 indicate that these species-specific primers could reliably identify this species. [Results]  The results suggest that the species-specific primers developed produce clear, unique and reproducible target DNA bands from individual adult wasps. Molecular identification of Q. erythrinae using species-specific primers from the rDNA ITS1 and ITS2 region is therefore feasible. [Conclusion]  This study identified a possible avenue for the molecular identification of Q. erythrinae.
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