Occurrence and prevalence of six bee viruses in Beijing

Author of the article:JIA Hui-Ru1** LIU Jin-Zu2 WANG Xing2 WU Yan-Yan1 ZHOU Ting1***

Author's Workplace：1. Key Laboratory of Pollinating Insect Biology of Agriculture, Institute of Apiculture Research, Chinese Academy of AgricultureScience, Beijing 100093, China; 2. Beijing Apiculture Silkworm Administrative Centre, Beijing 100029, China

Key Words：bee viruses, multiplex RT-PCR, Israeli acute paralysis virus, deformed wing virus, sacbrood virus, black queen cell virus

Abstract：     [Objectives] To provide a theoretical basis for the prevention of bee virus diseases in Beijing. [Methods] Sixhoney bee viruses; Israel acute paralysis virus (IAPV), deformed wing virus (DWV), Sacbrood virus (SBV), acute beeparalysis virus (ABPV), black queen cell virus (BQCV) and chronic bee paralysis virus (CBPV) were surveyed usingmultiplex RT-PCR，and the specificity of the RT-PCR assay was confirmed by sequence analysis. [Results] IAPV was themost widespread virus with DWV was the second most prevalent. Chronic bee paralysis virus (CBPV) and acute beeparalysis virus (ABPV) were not detected. Simultaneous multiple infections of viruses were common in the testedsamples. [Conclusion] IAPV, DWV, SBV and BQCV are probably wide spread in the Beijing region.