[Objectives]  To investigate the effect of an experimental release of transgenic corn in JilinProvinceon soil fauna. [Methods]  Field experiments including two corn varieties (transgenic corn C63-1 and its Control) were conducted to investigate the effects of transgenic corn on soil Collembola in 2012. [Results]  Our results showed that density, species richness, diversity and community structure were not impacted by transgenic corn cultivation but some rare species disappeared in transgenic corn plots. Collembola community structure was significantly affected by different corn growth periods; Density of Collembola was highest in the mature corn stage, possibly because of abundant corn litter (a food resource of Collembola) during that growth stage. [Conclusion]  Transgenic corn cultivation did not have a significant effect on soil Collembola over the course of a one year experiment. However, longer-term, controlled experiments should be done to ascertain the environmental safety of transgenic corn cultivation.