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Issue:ISSN 2095-1353
           CN 11-6020/Q
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Your Position :Home->Past Journals Catalog->2014年51 No.5

The expression of serine proteinases (SPs) and serine protease inhibitor (Serpins) genes in silkworms, Bombyx mori, treated with hormones and subject to food restriction
Author of the article:GAN Li-Ping1** LIU Ren-Hua1 LONG Fei-Fei2 ZHANG Jin2 XU Shi-Qing2
Author's Workplace:1. College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404000, China; 2. National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China
Key Words:Bombyx mori, serine proteinases (SPs), Serine protease inhibitor (Serpins), hormone and food restriction treatment, gene expression
Abstract:

     [Objectives]  To investigate the effects of hormone treatment and food restriction on the gene expression of serine proteases (SPs) and serine protease inhibitors (Serpins) in Bombyx mori. [Methods]  mRNA expression and protein levels of SPs and Serpin genes were tested by RT-PCRReal time PCR and western blotin silkworms treated with 20-hydroxyecdysone (20E), juvenile (JHA) hormones and food restriction. [Results]  mRNA expression was consistent with protein levels. SPs were especially expressed in the midgut of mid-fifth instar, however Serpins were mainly expressed in the silk glands, achieving a higher level in the late fifth instar. SPs and Serpin genes displayed different regulatory trends in the fifth and third instar silkworm larvae treated with 20E and JHA. Hormone regulatory effect was mainly influenced by developmental stages and dosage. The expression of the Spi1 gene was down regulated after food restriction, in addition this process occurred later than for the Spp gene, which implies that food restriction directly affects Serpins. It also illustrates that the silk glands respond faster to food supply than the midgut. The tissue specificity of SPs and Serpins demonstrates that they participate in tissue-specific transcriptional regulation. Both SPs and Serpins were differentially expressed in different developmental stages in response to either hormone treatment or food restriction. [Conclusion]  These findings on the function and specificity of SP and Serpins further understanding of the mechanism of silk formation and accumulation.

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