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Issue:ISSN 2095-1353
           CN 11-6020/Q
Director:Chinese Academy of Sciences
Sponsored by:Chinese Society of Entomological;institute of zoology, chinese academy of sciences;
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Your Position :Home->Past Journals Catalog->2014年51 No.5

A bioassay suitable for screening pesticides to control the wireworm Agriotes spp.
Author of the article:LI Yao-Fa1** DANG Zhi-Hong1 GAO Zhan-Lin1 PAN Xiao-Hua2 PAN Wen-Liang1***
Author's Workplace:1. Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, IPM Center of Hebei Province, Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, P. R. China, Baoding 071000, China; 2. Plant Protection Station of Bazhou City, Bazhou 065700, China
Key Words: soil insect, wireworm, bioassay, insect dipping method, soil mixing medicine method, food dipping method
Abstract:

    [Objectives]  To explore appropriate pesticide bioassay methods for the wireworm. [Methods]  Toxicities of several kinds of pesticide to Agriotes fusicollis Miwa were determined by the insect dipping method, soil mixing method and food dipping method. The advantages and disadvantages of these three methods, including accuracy, regularity, repeatability and convenience, were compared and analyzed. Lastly, the prospects of applying the three bioassay methods was evaluated. [Results]  Each bioassay method had its advantages and disadvantages. Although the soil mixing method was relatively difficult and it was difficult to judge whether insects were alive or dead, it was more accurate than the others. The food dipping method was reliable requires a very high degree of experimental rigor with respect to food treatment. The insect dipping method, was relatively inaccurate, but was repeatable, simple and allowed easy assessment of whether insects were alive or dead. It also allowed a large number of insects to be treated at the same time. [Conclusion]  Although all three methods can be used to determine the toxicity of insecticides to wireworms, the insect dipping method should become the standard bioassay method. Different methods can be used depending on different experiment aims or the pesticides being tested.

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