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Your Position :Home->Past Journals Catalog->2015年52 No.1

Impact of cold storage on the pupal development and survivorship of Liriomyza sativae
Author of the article:ZHANG Jin-Yu1,2** GE Jin2 WEI Jia-Ning2***
Author's Workplace:1. School of Life Sciences, Anhui University, Hefei 230039, China; 2. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Key Words: Liriomyza sativae, low-temperature storage, eclosion rate, developmental morphology, bristle stage
Abstract: [Objectives]  Liriomyza sativae Blanchard, the vegetable leafminer, has been an important insect pest of vegetables and ornamentals since it was introduced in China in the1990’s and has also become a model for the study of plant-insect interactions, cold tolerance, and host adaptation. To improve its captive husbandry, we studied the effect of cold storage at its developmental threshold (8) on pupal development and survivorship. [Methods]  Three-day pupae of L. sativae were collected and stored at 8 and the effect of different periods (0, 7, 14, 21, 28 and 35 days) of cold storage on pupal development and survivorship monitored. The morphology of developing pupae was observed by dissection at 24 h intervals and pupae were categorized to different development stages on the basis of morphology. In order to determine the causes of mortality, we dissected and compared dead pupae from pupal stage and treatment. [Results]  Eclosion rates decreased with the duration of cold storage. Emergence rates significantly declined after 14 days, and fell to zero on day 35. Pupal development was divided into 7 stages; the prepupal, cryptocephalic, early phanerocephalic, yellow-eyed, amber, red-eyed, and bristle stages. We found that most mortality occurred in the bristle stage, and that the percentage of dead pupae increased with duration of cold storage. [Conclusion]  The optimal duration of cold storage for pupae was <14 days at 8 and the bristle stage of pupal development is the most sensitive to cold temperature.


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